Distribution and Classification of Dehydrins in Selected Plant Species Using Bioinformatics Approach

Document Type : Research Paper

Authors

1 Department of Biotechnology, University of Malakand, Chakdara, Lower Dir, Pakistan

2 Department of Biosciences, COMSAT University, Islamabad, Pakistan

3 Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan

4 Department of Biochemistry, Abdul Wali Khan University Mardan, Pakistan

Abstract

Background: Plant growth, reproduction and yields are severely damaged under adverse environmental stresses. These stresses can be either biotic or abiotic, and many stress related proteins are expressed in response to these stresses. Among these proteins dehydrins are reported to have a role primarily in the abiotic stresses. Dehydrins are very diverse proteins and a uniform annotation system is needed for their functional characterization in the future research.
Objectives: The aim of the present work is to identify, classify and analyze the expression of dehydrin proteins under different biotic and abiotic stresses in the selected plant species by using different computational tools.
Materials and Methods: Prosite database is used for dehydrin proteins identification, and to conform the location of conserved motifs in selected plant species. The dehydrins extracted from uniprot database were annotated, based on the ensemble plant gene id. Subcellular localization was predicted using PSI predictor tool. Dehydrin expression analyses were
retrieved form the genevestigator tool.
Results: Dehydrins were annotated on the basis of dehydrin gene locus and conserved motifs available in different domain databases. Dehydrins were identified and annotated in Arabidopsis thaliana (13), Glycine max (12), Zea mays (05), Oryza
sativa (11), Solanum tuberosum (05), Solanum lycopersicum (06), Triticum aestivum (32) and Vitis vinifera (06). It has been proposed that dehydrins are located primarily in cytosol and nucleus. Based on genevestigater expression analyses the plant species selected for this study contain all the classes of dehydrins, namely YnSKn, Kn, SKn, and YnKn; except class KnS.
Conclusions: Dehydrins are diverse proteins and a uniform classification is introduced for their better characterization. The distribution of dehydrins in different tissues and developmental stages suggest an important function throughout plant
growth cycle. It has also been concluded that dehydrins expressed particularly in drought, cold and salt stresses, and may have limited role in heat, anoxia, heavy-metal and biotic stresses as well.

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Iranian Journal of Biotechnology
Volume 18, Issue 4
December 2020
Pages 56-66

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