Biosynthesis, Characterization, and Antibacterial Activity of Silver Nanoparticles Derived from Aloe barbadensis Miller Leaf Extract

Document Type: Research Paper


1 Department of Biochemistry, University of Karachi, Karachi –75270, Pakistan

2 Department of Biotechnology, University of Karachi, Karachi – 75270, Pakistan

3 Department of Biochemistry, University of Karachi, Karachi, 75270, Pakistan



Background: There is a growing commercial demand for nano-formulations due to their prevalence applicability in various areas of bio-nanotechnology. Numerous chemical and physical methods have traditionally been used to synthesize silver nanoparticles, but they are limited due to use of toxic and harmful chemicals, thus drew researchers’ attention towards the biosynthesis of the silver nanoparticles by using medicinal plant.
Objective: The present study enlightens the synthesis of silver nanoparticles in an echo-accommodating way by using aqueous Aloe vera leaf extract (AVLE) and evaluate its antimicrobial potential.
Materials and Methods: The synthesis of silver nanoparticles using AVLE was determined by UV–vis spectrum and SEM. The optimization of different reaction conditions was measured, and antibacterial activity was evaluated by the disc diffusion method.
Results: The optimum synthesis of AV-AgNPs showed at a 1mM concentration of silver nitrate, 5:95 ratio of AVLE to silver nitrate solution, pH 8 at ambient temperature for 24 hours. The synthesis was confirmed by UV–Vis spectroscopy maximum absorbance at 400 nm while SEM showed spherical morphology with an average particle size 20-24 nm. The antibacterial activity of AV-AgNPs was measured by disc diffusion method and exhibits significant antibacterial activity against both gram-positive and gram-negative bacteria.
Conclusion: This method appears promising for the biosynthesis of silver nanoparticles by using Aloe vera with potent bactericidal activity, thus suggesting its role in clinical therapeutics and other fields.


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