Document Type : Research Paper
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Department of Biology, University of Guilan, Rasht, Iran
Department of Biology, University of Guilan, Rasht, Iran
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Department of Plant Protection Research, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran.
Plant Protection Department, Rice Research Institute of Iran (RRII), Rasht, Iran
Background: MicroRNAs, as small non-coding RNAs, are recently reported to be involved in plant defense system against pathogens including fungi.
Objective: In this research, it was intended to investigate candidate susceptible rice (Oryza Sativa) Osa-miRNA expression alteration following the infection by Rhizoctonia solani.
Materials and Methods: To this aim, literature review suggested eight conserved plant miRNAs that are involved in other plant-pathogen interactions. Then, sixty days old rice plants (Hashemi, susceptible cultivar) were inoculated with R. solani and candidate miRNA expression alterations were investigated 2 hpi (hours post inoculation), 2 dpi (days post inoculation) and 6 dpi.
Results: RT-qPCR analysis suggested four subgroups of candidate miRNAs based on the time of their responses to the pathogenesis of R. solani. While Osa-miR-156 was early-responsive, Osa-miR159 was the last-responsive and Osa-miR167, Osa-miR171, Osa-miR408, and Osa-miR444 were late responsive to R. solani infection. Osa-miR166 and Osa-miR393 were non-responsive to this infection, compared to the mock-inoculated control group. Consistently, Os-SPL3 and Os-MADS known target genes were expressed in reverse correlation to Osa-miR156 and Osa-miR444, respectively.
Conclusions: From these data, it is suggested that both early (Osa-miR-156) and late (Osa-miR167, Osa-miR171, OsamiR408, Osa-miR444) responsive miRNAs might be involved in R. solani infection in rice plants.