Document Type: Brief Report
PhD of Nanobiotechnology, Polymerization Department, Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Genetics Department, Biological Science, Tarbiat Modares University, Tehran, Iran.
3- PhD of Nanobiotechnology, Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
PhD of Polymerization, Polymerization Department, Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Background: Dynamic light scattering (DLS) and electron microscopy (EM) are the most practical techniques for nanoparticles (NPs) characterization. However, the impediments which involved the sample preparation method lead to failure in provided results of mentioned device analysis. These problems will be intensifying, if the examined samples are the soft nanocarriers such as organic ones or biological samples.
Objectives: In order to achieve the appropriate results from DLS and EM analysis, an optimized protocol was introduced by this research which would prepare samples with high degree of quality and accuracy.
Materials and methods
Morphological analysis of prepared polymeric nanocarriers (micelles, nanogels) by this protocol were done. Filtration, dilution and sonication as three crucial and effectiveness steps of sample preparation were assessed through DLS data and EM images.
Results: This research has tried to introduce a facile method with novelty of simplicity and rapidity. These triple steps could improve the quality of morphological data. The obtained results indicated that sample preparation methods have the most effective factors on sample size distribution and homogeneity of desired samples.
Conclusion: The suggested optimized preparation method will be helpful for all soft nanomaterial’s samples.