“Evaluation of Gelatinolytic and Collagenolytic Activity of Fasciola hepatica Recombinant Cathepsin-L1” Kobra Mokhtarian, Reza Falak, Zahra Heidari

Document Type: Short Note

Authors

1 Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran

3 Department of Medical Parasitology, School of Medicine, Ardabil University of Medical Sciences

10.30498/ijb.2020.143160.2357

Abstract

Background: Cysteine proteases of the liver fluke, Fasciola hepatica, participate in catabolism of proteins, migration of the fluke through host tissues and combating against host immune system.
Objectives: In this study we evaluated proteolytic activity of F. hepatica recombinant cathepsin L1 (rCL1) against gelatin and collagen as common substrates.
Material and Methods: The coding sequences of F. hepatica CL1 was cloned and expressed in E. coli, in our previous study. The rCL1 was purified by nickel affinity chromatography with a His Trap Column. The protein concentrations of the purified fractions were determined by Bradford assay. Rat collagen type-1 was treated with distinct amounts of rCL1 at 37°C, overnight, and the byproduct was analyzed by SDS-PAGE. Furthermore, we used bovine skin gelatin as zymography susbtrate to evaluate the gelatinolytic activity of the purified rCL1.
Results and conclusion: Recombinant CL1 was capable to digest intact type-1 collagen within 24 h and the gelatinlytic activity of rCL1 was visible at approximately 37 kDa region, with optimal activity at acidified conditions (pH 4). Findings provide a possible mechanism by which a major secretory molecule of F. hepatica could be involved in parasite survival as well as its pathogenesis.

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