Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs. Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies, to target pathogenic E. coli surface antigens. Material and methods: We immunized a camel with a killed strain we had previously isolated from a diarrheic camel calf and identified as expressing the F17 fimbriae antigen. Results: The immunized animal developed an anti-E.coli immune response including heavy-chain antibodies. Lymphocytes from this animal were purified and RNA isolated to create a VHH library by phage display with a size of about 109 individual transformants. Panning on live E. coli cells resulted in the isolation of VHH fragments specific to the cell surface antigens. Conclusion: The identification of these antigens can lead to the development of new diagnostic and therapeutic tools against diarrhea.
Salhi, I., Bessalah, S., Snoun, D., Khorchani, T., & Hammadi, M. (2020). Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary. Iranian Journal of Biotechnology, 18(1), 16-23. doi: 10.30498/ijb.2020.127753.2247
MLA
Imed Salhi; Salma Bessalah; Dalila Snoun; Touhami Khorchani; Mohamed Hammadi. "Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary". Iranian Journal of Biotechnology, 18, 1, 2020, 16-23. doi: 10.30498/ijb.2020.127753.2247
HARVARD
Salhi, I., Bessalah, S., Snoun, D., Khorchani, T., Hammadi, M. (2020). 'Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary', Iranian Journal of Biotechnology, 18(1), pp. 16-23. doi: 10.30498/ijb.2020.127753.2247
VANCOUVER
Salhi, I., Bessalah, S., Snoun, D., Khorchani, T., Hammadi, M. Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary. Iranian Journal of Biotechnology, 2020; 18(1): 16-23. doi: 10.30498/ijb.2020.127753.2247