Document Type: Research Paper
Bam University of Medical Sciences, Bam, Iran.
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Nanobioelectrochemistry Research Center, Bam University of Medical Sciences, Bam Iran
Clinical Research Center, Pastor Educational Hospital, Bam University of Medical Sciences, Bam, Iran
Behbahan Faculty of Medical Sciences, Behbahan, Iran
Department of Clinical Microbiology, Iranshahr University of Medical Sciences, Iranshahr, Iran
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
Regional Centre of Advanced Technologies and MaterialsFaculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic
Background: Nanoparticles (NPs) with unique physical and chemical properties can be used for therapeutic purposes because of their strong antimicrobial activates. Nanoparticles have been used as active agents to inhibit microorganisms for therapeutic purposes.
Objectives: In view of the strong antimicrobial activity of nanoparticles, the biogenic synthesis and leishmanicidal activity of rod-shaped zinc oxide (R-ZnO) nanoparticles was explored using Lilium ledebourii tuber extrct.
Materials and Methods: The ensuing nanoparticles are characterized by UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction and their leishmanicidal activity evaluated against the Leishmania major (L. major) by MTT assay.
Results: The R-ZnO nanoparticles displayed excellent leishmanicidal activity against the L. major as they significantly inhibited the multiplication rate of intramacrophage amastigotes in a dose-dependent manner under in vitro conditions. The IC50 values of R-ZnO nanoparticles being ~ 0.001 mg/mL. R-ZnO nanoparticles can inhibit L. major growth in a dose-dependent manner under in vitro conditions.
Conclusion: A simple, low-cost feasible and eco-friendly procedure was developed for biosynthesis of R-ZnO nanoparticles using natural bioresource that can inhibit human parasite cells growth in a dose-dependent manner under in vitro conditions.