Detection of Single-Nucleotide Polymorphism in the Bovine AGPAT6 Gene Associated with Milk Fat Content using Tetra-Primer ARMS PCR-based Assay in Karan Fries Breeding Bulls

Document Type: Brief Report

Authors

1 Krishi Vigyan Kendra, Jhansi, Banda University of Agriculture and Technology, Banda

2 Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India

3 Animal Biotechnology Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India

4 Animal Genetics and Breeding Division, Tamil Nadu Veterinary and Animal Sciences University, Chennai- 600051, Tamil Nadu, India

Abstract

Background: The bovine AGPAT6 gene is one of the potential candidate genes governing milk fat synthesis.
Objectives: Identification of single nucleotide polymorphisms (SNP) in the targeted region of AGPAT6 gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were sought.
Materials and Methods: A tetra-primer ARMS PCR technique was adapted to genotype an SNP, g.36,175,805C>T located on 5’ flanking region of AGPAT6 gene. The relationship between EBV of milk production traits and polymorphic locus of AGPAT6 gene was assessed.
Results: Three kinds of genotype (CC, CT, and TT) with respect to g.36,175,805C>T SNP locus were observed. The identified SNP had significant (P

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