TY - JOUR ID - 9426 TI - Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Falahatpishe, Hamidreza AU - Jalali, Mahmoud AU - Badami, Naser AU - Mardani, Nadia AU - Khosravi-Darani, Kianoush AD - Department of Food Technology Research, National Nutrition and Food Technology Research Institute, Shahid Beheshti University, M.C., P.O. Box 19395-4147, Tehran, I.R. Iran AD - Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran Y1 - 2007 PY - 2007 VL - 5 IS - 2 SP - 110 EP - 113 KW - Alkalophilic Bacillus KW - Protease KW - Process variables KW - Purification DO - N2 - This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50ÂșC. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) at pH>10 UR - https://www.ijbiotech.com/article_9426.html L1 - https://www.ijbiotech.com/article_9426_f4e408bdf8aee17bd08445f937d29917.pdf ER -