TY - JOUR ID - 85181 TI - Investigation of Desulfurization Activity, Reusability, and Viability of Magnetite Coated Bacterial Cells JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Bardania, Hassan AU - Raheb, Jamshid AU - Arpanaei, Ayyoob AD - Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran AD - Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AD - Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Y1 - 2019 PY - 2019 VL - 17 IS - 2 SP - 14 EP - 20 KW - Rhodococcus erythropolis KW - Nanoparticles KW - Equipment Reuse DO - 10.21859/ijb.2108 N2 - Background: Magnetic separation using magnetic nanoparticles can be used as a simple method to isolate desulfurizing bacteria from a biphasic oil/water system. Objectives: Magnetite nanoparticles were applied to coat the surface of Rhodococcus erythropolis IGTS8 and Rhodococcus erythropolis FMF desulfurizing bacterial cells, and the viability and reusability of magnetite-coated bacteria evaluated by using various methods. Material and Methods: Magnetite nanoparticles were synthesized through a reverse co-precipitation method. Glycine was added during and after the synthesis of magnetite nanoparticles to modify their surface and to stabilize the dispersion of the nanoparticles. The glycine-modified magnetite nanoparticles were immobilized on the surface of both oil-desulfurizing bacterial strains. Reusability of magnetite-coated bacterial cells was evaluated via assessing the desulfurization activity of bacteria via spectrophotometry using Gibb's assay, after the separation of bacterial cells from 96h-cultures with the application of external magnetic field. In addition, CFU and fluorescence imaging were used to investigate the viability of magnetite-coated and free bacterial cells. Results: TEM micrographs showed that magnetite nanoparticles have the size approximately 5.35±1.13 nm. Reusability results showed that both magnetite-coated bacterial strains maintain their activity even after 5 × 96h-cycles. The viability results revealed glycine-modified magnetite nanoparticles did not negatively affect the viability of two bacterial strains R. erythropolis IGTS8 and R. erythropolis FMF. Conclusions: In conclusion, the glycine-modified magnetite nanoparticles have great capacity for immobilization and separation of desulfurizing bacteria from suspension. UR - https://www.ijbiotech.com/article_85181.html L1 - https://www.ijbiotech.com/article_85181_5fc3800f79a11b1fd52f367067a3dbc7.pdf ER -