TY - JOUR ID - 85179 TI - Purification, Characterization and Thermodynamic Assessment of an Alkaline Protease by Geotrichum Candidum of Dairy Origin JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Muhammad, Abubakar AU - Syed Ali Imran, Bokhari AU - Jean-Paul, Vernoux AU - Muhammad, Ishtiaq Ali AU - Faryal, Rani AU - Nathalie, Desmasures AU - Muhammad, Imran AD - Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan AD - Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan AD - Aliments Bioprocédés Toxicologie Environnement (ABTE), E.A. 4651, Université de Caen Basse-Normandie, Esplanade de la Paix, CAEN Cedex, France AD - Aliments Bioprocédés Toxicologie Environnement (ABTE), E.A. 4651, Université de Caen Basse-Normandie, Esplanade de la Paix, CAEN Cedex, France Y1 - 2019 PY - 2019 VL - 17 IS - 2 SP - 30 EP - 37 KW - Geotrichum candidum KW - Alkaline Serine Protease, Kinetics KW - Thermodynamics DO - 10.21859/ijb.2042 N2 - Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi. Material and Methods: In total twelve G. candidum strains were screened for their proteolytic activity by using standard protease assay. The protease production from G. candidum QAUGC01 was optimized by varying physio-chemical conditions. The protease was purified by using two-step method: ammonium sulfate precipitation and gel filtration chromatography. Protease was further characterized by studying various parameter like temperature, pH, modulators, metal ions and organic solvent. A thermodynamic study was also carried out to explore the half-life of protease. Results: The G. candidum grew profusely at 25 °C and at an initial pH of 4.0 for 72 h of incubation producing 26.21 U/mlmaximum extracellular protease. Protease revealed that Vmax and Km was 26.25 U.ml-1.min-1 and 0.05 mg.mL-1, respectively using casein as substrate. The enzyme was stable at a temperature range (25-45 ºC) and pH (8-9). Residual enzyme activity was strongly inhibited in the presence of PMSF (7.5%). The protease could hydrolyze proteinaceous substrates, casein (98%) and BSA (95%). The thermodynamic studies explored that the half-life of the enzyme that was 106.62 min, 38.72 min and 15.71 min at 50, 60 and 70 ºC, respectively. Conclusions: Purified protease from G. candidum GCQAU01 is an ideal candidate for industrial application. UR - https://www.ijbiotech.com/article_85179.html L1 - https://www.ijbiotech.com/article_85179_a1504d975c737e39ee7ab35c31c35fd4.pdf ER -