TY - JOUR ID - 85172 TI - Gene-Based Marker to Differentiate Among B, A, and R Lines in Hybrid Production of Rapeseed Ogura System JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Akbari Afjani, Javad AU - Soltani Najafabadi, Masood AU - Gholi Mirfakhraei, Reza AD - Department of Biotechnology, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran AD - Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension (AREEO), Shahid Fahmideh BLVRD, Seed and Plant Improvement Institute Campus, Karaj, Iran AD - Department of Plant Genetics and Breeding, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran Y1 - 2019 PY - 2019 VL - 17 IS - 3 SP - 50 EP - 54 KW - Biomarkers KW - Brassica rapa, Rf Allele DO - 10.29252/ijb.1870 N2 - Background: In plant breeding program to produce hybrid varieties, pair of male sterile and restorer fertility lines are required. Differentiation of lines possessing restorer fertility allele from the lines lacking it remove the need for the progeny test, and thus reducing the time and the cost in the hybrid production procedure. Canola breeding program in Iran has concentrated toward production of domestic hybrid varieties, however, it suffers from lack of molecular information in restore fertility status of lines, and therefore it needs time and tedious activities. Objectives: To design gene-based markers for distinguishing R-, A- lines and hybrids in sunflower breeding programs. Material and Methods: Aligning sequences of locus responsible for male sterility and that of male fertility resulted in finding differences in the loci, which used to define two set of suitable primer pairs. Genomic DNA from 25 R-lines (23 inbred lines and two commercial lines), 9 A-lines (7 inbred lines and two commercial lines), one B-line and two commercial hybrids were extracted and used in PCR as template. Results: Using one-primer pairs, a band of nearly 1500 bp was amplified in restorer lines but not in A-, B- lines. Another primer pair used to distinguish hybrids (heterozygout) from restorer lines. Results of the report is predicted to be used in canola breeding for hybrid production. Conclusions: Although the molecular bases for the male sterility and fertility restoration in rapeseed is not published, taking advantages of gene-based markers, make rapeseed breeding program more efficient regarding time and costs. UR - https://www.ijbiotech.com/article_85172.html L1 - https://www.ijbiotech.com/article_85172_55da68799eb9a32c98d0de48aeabe185.pdf ER -