TY - JOUR ID - 7249 TI - Production and Purification of Equine Chorionic Gonadotropin Hormone Using Polyclonal Antibody JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Sharif, Mohammad Ali AU - Kohram, Hamid AU - Zare Shahneh, Ahmad AU - Zolfagharian, Hossein AU - Abedi Kiasari, Bahman AU - Hedayati, Mehdi AD - Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R. Iran AD - Razi Vaccine & Serum Research Institute, Karaj, I.R. Iran AD - Cellular & Molecular Research Center, Research Institute for Endocrine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran Y1 - 2014 PY - 2014 VL - 12 IS - 1 SP - 30 EP - 34 KW - eCG, Ouchterlony KW - Polyoclonal Antibody KW - Solid-Phase Extraction (SPE) DO - 10.5812/ijb.15137 N2 -   Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Objectives: The aim of the current study was to develop an accurate and fast method for production and purification of eCG using polyoclonal antibody assay. Materials and Methods: The blood samples (300 ml) were taken from jugular vein of 17 mares on days 50, 70 and 90 of pregnancy. Plasma of the samples was siphoned and phenol solution has been added to the plasma and stored in the refrigerator until eCG extraction. To prepare polyoclonal antibody against eCG, four male rabbits, about 4-months-old and 2-kg weights, were chosen. A basic immunization was done by injecting 25 IU of eCG to the rabbits. Ouchterlony assay or double immunodiffusion test was used to assess the immunization and the titer of antiserum against eCG. eCG has been purified from the plasma via Solid-Phase Extraction (SPE) method. Results: Results based on agarose-gel double immunodiffusion test showed that rabbits have been completely immunized. SDS-PAGE analysis showed purified eCG is extracted without any significant contamination. Conclusions: The extraction of eCG with polyclonal antibody using SPE method and production of anti- eCG antiserum in rabbits is suitable and may be a cost effective method for large scale production of eCG and anti- eCG antiserum in Iran. UR - https://www.ijbiotech.com/article_7249.html L1 - https://www.ijbiotech.com/article_7249_5994bc5ee9d68374bd7eec63043fa862.pdf ER -