TY - JOUR ID - 7111 TI - Bacterial overexpression of the human interleukin-2 in insoluble form via the pET Trx fusion system JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Boozarpour, Sohrab AU - Sadeghizadeh, Majid AU - Shokrgozar, Mohammad Ali AU - Hosseinkhani, Saman AU - Shojaosadati, Seyed Abbas AU - Gharavi, Sara AU - Ahangari, Ghasem AU - Ranjbar, Bijan AD - Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-154, Tehran, I.R. Iran. AD - National Cell Bank of Iran, Pasteur Institute, P.O. Box 1316943551, Tehran, I.R. Iran. AD - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115- 154,Tehran, I.R. Iran. AD - Biotechnology Group of Chemical Engineering, Faculty of Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, I.R. Iran. AD - Department of Biology, Alzahra University, P.O. Box 1993891176, Tehran, I.R. Iran. AD - Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, I.R. Iran. AD - Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-154, Tehran, I.R. Iran. Y1 - 2010 PY - 2010 VL - 8 IS - 4 SP - 270 EP - 274 KW - Human IL-2 KW - Soluble protein KW - Inclusion body KW - Overexpression KW - E. coli DO - N2 - Selection of a system for successful recombinant protein production is important. The aim of this study wasto produce high levels of human interleukin-2 (hIL-2) in soluble form. To this end, the pET32a vector inEscherichia coli BL21 (DE3) was used as an expression system, since it was previously used for the productionof mouse IL-2 in soluble form. The results indicated that contrary to expectations, the expressed proteinwas in the form of inclusion bodies and perhaps amino acid differences between human and mouse IL-2 should be determinant. The hIL-2 protein is a small peptide, therefore its recovery as a biologically functional protein by the process of refolding may be feasible and could lead to high yields at the industrial scale. UR - https://www.ijbiotech.com/article_7111.html L1 - https://www.ijbiotech.com/article_7111_7967f761c8f5942c6967289c5fb76165.pdf ER -