TY - JOUR ID - 6994 TI - Calpastatin Polymorphism and Its Association with Daily Gain in Kurdi Sheep JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Nassiry, Mohammad Reza AU - Tahmoorespour, Mojtaba AU - Javadmanesh, Ali AU - Soltani, Mahdi AU - Foroutani Far, Saheb AD - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Y1 - 2006 PY - 2006 VL - 4 IS - 3 SP - 188 EP - 192 KW - Calpastatin KW - Polymorphism KW - Kurdi Sheep KW - daily gain DO - N2 - Association of genetic polymorphism in the calpastatin (CAST) gene with average daily gain was examined in Iranian purebred Kurdi sheep. The genotypes for CAST were determined by the PCR-SSCP method. Blood samples were collected from 84 purebred Kurdi sheep belonging to the Kurdi Breeding Station located in the Khorasan province, north-east of Iran. Extraction of genomic DNA was based on the Guanidinium Thiocyanate-Silica gel method. Three genotypes including aa, ab and ac with frequencies of 0.55, 0.32 and 0.13, were observed in this population. Chi-square test confirmed Hardy-Weinberg equilibrium for the CAST loci. Average heterozygosity (37%) of  the CAST locus for Kurdi sheep was slightly low. Daily gain birth to weaning (GBW); weaning to six months (GWS); six to nine months (GSN) and nine to yearling (GNY) were analyzed by a statistical model comprising SSCP (Single strand conformation polymorphism) and significant effect (P<0.05) of CAST genotypes was observed for GBW only. UR - https://www.ijbiotech.com/article_6994.html L1 - https://www.ijbiotech.com/article_6994_40ef600d702f11adb0fa67de34e0367d.pdf ER -