TY - JOUR ID - 6937 TI - CpG Motif as an Adjuvant in Immunization of a Recombinant Plasmid Encoding Hepatitis C Virus Core Protein JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Karami, Ali AU - Sarbolouki, Mohammad Nabi AU - Khatami, Fatemeh AU - Rastgoo, Nasrin AD - Department of Molecular Medicine, Research Center of Molecular Biology, Institute of Military Medicine, Bagyatallah University of Medical Science, Tehran, I.R. Iran. AD - Institute of Biophysics and Biochemistry, University of Tehran, P.O.Box: 13145-1384 Tehran, I.R. Iran. AD - Department of Immunology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran. AD - Department of Immunology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R. Iran. Y1 - 2005 PY - 2005 VL - 3 IS - 1 SP - 64 EP - 66 KW - CpG Motif KW - Dendrosome, Hepatitis C Virus KW - HCV Core pcDNA3 DO - N2 - The immunogenicity and protective efficacy of DNA vaccines have been demonstrated in numerous animalmodels of infectious diseases. In order to increase the potency of DNA vaccines, in this study, conventionaladjuvants such as aluminium phosphates, dendrosome, CpG motif and mixture of aluminium phosphateand CpG motif have been tested. Female BALB/c mice were immunized with mixture of 10, 25 and 50 μg HCVcore pcDNA3. Each dose of recombinant pcDNA3 together with different adjuvants used as an immunogenwere injected three times on day; 0, 30 and 50 days. Blood samples were collected at four different times intervals and antibody response against HCV core antigen was determined by HCV core ELISA kit. The results indicate that the best antibody response was with mixture of aluminium phosphate and CpG motif as an adjuvant. This data suggest that the antibody response induced following DNA immunization can be modified by formulation strategies. UR - https://www.ijbiotech.com/article_6937.html L1 - https://www.ijbiotech.com/article_6937_83cb3be0e1734bdb3683703fdbc8fadc.pdf ER -