TY - JOUR ID - 6924 TI - Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Khalilzadeh, Rasoul AU - Shojaosadati, Seyed Abbas AU - Bahrami, Ali AU - Maghsoudi, Nader AD - Biotechnology Group, Chemical Engineering Department, Engineering Faculty, Tarbiat Modarres University, P.O. Box 14155-143, Tehran, I.R. Iran. AD - Neuroscience Research Center, Shaheed Beheshti University, P.O. Box: 19835- 181. Tehran, I.R. Iran. Y1 - 2004 PY - 2004 VL - 2 IS - 2 SP - 113 EP - 122 KW - Human interferon-gamma KW - Fed-batch cultivation KW - recombinant E. coli KW - Specific growth rate KW - Glucose KW - Glycerol DO - N2 - A simple fed-batch process with pre-determined exponential feeding strategy for high-cell-density cultivationof recombinant E. coli BL21 (DE3) in defined medium was developed. In this feeding method glucose andglycerol were used as the sole sources of carbon and energy to increase the cell density exponentially atcontrolled specific growth rates, which do not cause the accumulation of acetate. Thus, sophisticated feedbackcontrol or extra equipment to prevent the accumulation of toxic level of acetate is not necessary. Thefinal cell densities of 100 and 118 gl-1 of dry cell mass for recombinant E. coli producing human interferon-γ(hIFN-γ) were obtained by using glucose and glycerol, respectively. The concentration of acetate was alwaysmaintained below toxic level. The specific yield of hIFN-γ with glucose and glycerol was 93 and 92 mgg-1of dry cell mass, and the overall productivity of hIFN-γ was 0.16 and 0.14 gl-1h-1 for these two carbon sources, respectively. UR - https://www.ijbiotech.com/article_6924.html L1 - https://www.ijbiotech.com/article_6924_9108db3709b7ac963427a974bbfd0c09.pdf ER -