TY - JOUR ID - 6877 TI - Study of Desulfurization Rate in Rhodococcus FMF Native Bacterium JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Akbarzadeh, S AU - Raheb, J AU - Aghaei, A AU - Karkhane, AA AD - National Research Center for Genetic Engineering and Biotechnology (NRCGEB) AD - National Research Center for Genetic Engineering and Biotechnology (NRCGEB), Y1 - 2003 PY - 2003 VL - 1 IS - 1 SP - 36 EP - 40 KW - Desulfurization KW - Dibenzothiophene KW - Rhodococcus DO - N2 - The Rhodococcus FMF (R. FMF) native bacterium was isolated from soil contaminated with oil in Tabrizrefinery. This bacterium carries three genes sox (dszA, B, C) on its genomic DNA. Preliminary studieshave proved that R. FMF strain possess desulfurization activity. In this work soxA and B genes wereamplified by PCR, after designing a pair of suitable primers. Analysis of PCR products on agarose gelelectrophoresis showed a sharp band in the 2.46 kb region, belonging to soxA and B genes. In addition,dot blotting using the sox4 probe, confirmed the presence of sox operon in the PCR product. The standardGibbs test was designed for desulfurization activity assay in which production of 2HBP (μM) in four bacterialstrains [R. FMF, P. aeruginosa (pESOX4), E.coli DH5α (pESOX3) and E. coli CC118λpir (pESOX4)]was recognized and compared. After 41h of dibenzothiophene (DBT) addition, Pseudomonas aeruginosa(pESOX4) produced 4.8 μM 2HBP and the local R. FMF produced 3.8 μM of DBT. Comparison of 2HBP production in the standard strain of P. aeruginosa (pESOX4) with the isolated R. FMF strain fromTabriz refinery revealed that the later is equally capable of desulfurizing DBT. UR - https://www.ijbiotech.com/article_6877.html L1 - https://www.ijbiotech.com/article_6877_c103cca839412e73379807a1a4fe1863.pdf ER -