TY - JOUR ID - 54475 TI - Sustained Release of Green Tea Polyphenols from Liposomal Nanoparticles; Release Kinetics and Mathematical Modelling JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Prakash Upputuri, Ravi Theaj AU - Azad Mandal, Abul Kalam AD - School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India Y1 - 2017 PY - 2017 VL - 15 IS - 4 SP - 277 EP - 283 KW - Anomalous transport KW - Diffusion KW - Erosion KW - sustained release KW - Zero order kinetics DO - 10.15171/ijb.1322 N2 - Background: Green tea polyphenols (GTP) are known to have several health benefits. In spite of these benefits, its application as a therapeutic agent is limited due to some of its limitations such as stability, bioavailability, and biotransformation. To overcome these limitations, liposomal nanoparticles have been used as a carrier of the GTP. Objective: Encapsulation of GTP to the liposomal nanoparticles in order to achieve a sustained release of the GTP and to determine the drug release kinetics and the mechanism of the release. Materials and Methods: GTP encapsulated liposomal nanoparticles were prepared using phosphatidyl choline and cholesterol. The synthesized particles were characterized for their particle size and morphology. In vitro release studies were carried out, followed by drug release kinetics, and determining the mechanism of release. In vitro, antioxidant assay was determined following 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: Atomic force microscope (AFM) and high resolution scanning electron microscope (HR SEM) images showed spherical particles of the size of 64.5 and 252 nm. An encapsulation efficiency as high as 77.7% was observed with GTP concentration of 5 mg.mL-1. In vitro release studies showed that the loading concentrations of GTP were independent to the cumulative percentage of the drug release. GTP release by varying the pH and temperature showed a direct correlation between the release parameter and the percentage of drug release. The higher the pH and temperature, the higher was the percentage of the drug release. The release data showed a good correlation with Zero order kinetics and the mechanism of the release being anomalous mode. Radical scavenging activity of the released GTP showed a potent scavenging activity. Conclusion: GTP encapsulated liposomal nanoparticles could be used as a delivery vehicle for achieving a sustained release.   UR - https://www.ijbiotech.com/article_54475.html L1 - https://www.ijbiotech.com/article_54475_1a57ec3b4600e65675cef5ec174baeeb.pdf ER -