TY - JOUR ID - 13565 TI - Effect of Antioxidants, Amino Acids and Plant Growth Regulators on in vitro Propagation of Rosa centifolia JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Akhtar, Gulzar AU - Jaskani, Muhammad Jafar AU - Sajjad, Yasar AU - Akram, Ahsan AD - Department of Horticulture, University College of Agriculture, University of Sargodha, Sargodha, Pakistan AD - Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan AD - Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan Y1 - 2016 PY - 2016 VL - 14 IS - 1 SP - 51 EP - 55 KW - Activated charcoal KW - Browning KW - In vitro Propagation KW - Rosa centifolia DO - 10.15171/ijb.1152 N2 - Background: Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. Objectives: The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Materials and Methods: Axillary buds of R. centifolia were sterilized with 70% ethyl alcohol for 4 min and 5% sodium hypochlorite for 2 min followed by three washing with sterilized double distilled water. In order to control oxidative browning, Ascorbic acid (100 mg.L-1), citric acid (100 mg.L-1) and activated charcoal (3 g.L-1) were used while to control withering of shoots, different concentrations (3.0 mg.L-1, 6.0 mg.L-1, 9.0 mg.L-1) of either glutamine, asparagine and proline were put into trial. Different concentrations of Benzyl aminopurine (BAP) and naphthalene acetic acid (NAA) were used for in vitro shoot and root formation. Results: Minimum browning percentage (20%) was achieved in the presence of activated charcoal (3.0 g.L-1) and pretreatment of explants with running tap water. Asparagin (9.0 mg.L-1) produced maximum shooting (93%), minimum withering (6.67%), and it took longer period (27 days) for shoots to wither. BAP (3.0 mg.L-1) + NAA (0.5 mg.L-1) was produced the highest number of shoots (1.63), in a shortest periods (9 days). For root production, NAA (1.5 mg.L-1) + BAP (0.5 mg.L-1) reduced the time to 11 days with maximum number of roots (4.33) and root length (4.20 cm). Conclusions: The supplement of activated charcoal (3.0 g.L-1), a sparagin (9.0 mg.L-1) and combination of BAP and NAA in the MS medium is effective for in vitro propagation of R. centifolia. UR - https://www.ijbiotech.com/article_13565.html L1 - https://www.ijbiotech.com/article_13565_e7ebe2f731c3079579edc8f9b9855d08.pdf ER -