TY - JOUR ID - 129285 TI - Expressing of Recombinant VEGFR2-specific Nanobody in Baculovirus Expression System JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Shokrollahi, Narjes AU - Habibi Anbouhi, Mahdi AU - Jahanian-Najafabadi, Ali AU - AliRahimi, Ehsan AU - Behdani, Mahdi AD - Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran AD - National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran AD - Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran Y1 - 2021 PY - 2021 VL - 19 IS - 1 SP - 60 EP - 66 KW - Angiogenesis KW - Baculovirus Expression System KW - Nanobody KW - SF9 insect cell DO - 10.30498/IJB.2021.2783 N2 - Background: Baculovirus expression system, introduced more than 20 years ago, is considered as a useful tool for large and complex eukaryotic recombinant protein production. A baculovirus expression vector is a recombinant virus which desired foreign protein coding sequences is under control of the virus gene promoter. Baculovirus only infects insect cells and do not normally infect vertebrates therefore, they possess no risk of biological risks for human. Objectives: The aim of this study was to recombinant expression of vascular endothelial growth factor (VEGF) reseptor-2 specific Nanobody in the baculovirus expression system. Materials and Methods: Gene of specific Nanobody against the VEGF reseptor-2 that called 3VGR19 was cloned and expressed in baculovirus system. Results: 3VGR19 Nanobody gene was amplified by Polymerase Chain Reaction (PCR) using the specific primers, and was cloned in pFastBac HTA plasmid. DH10Bac bacteria was transformed with resulted donor plasmid. The cultured Sf9 insect cell line was transfected with recombinant bacmid, and finally, the expression and purification of 3VGR19 was confirmed in insect cells. Conclusions: In conclusion, Transient infection of insect cells with baculovirus can be a promising technology for expression of antibody fragments. UR - https://www.ijbiotech.com/article_129285.html L1 - https://www.ijbiotech.com/article_129285_d10021371e2aa09e7107660981d74709.pdf ER -