TY - JOUR ID - 111374 TI - Expression Alteration of Candidate Rice MiRNAs in Response to Sheath Blight Disease JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Talesh Sasani, Soheila AU - M. Soltani, Bahram AU - Mehrabi, Rahim AU - Samavatian, Hossein AU - Padasht-Dehkaei, Fereidoun AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Department of Biology, University of Guilan, Rasht, Iran AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. AD - Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran AD - Department of Plant Protection Research, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran. AD - Plant Protection Department, Rice Research Institute of Iran (RRII), Rasht, Iran Y1 - 2020 PY - 2020 VL - 18 IS - 4 SP - 39 EP - 46 KW - miRNA KW - Rhizoctonia solani KW - Rice plant DO - 10.30498/IJB.2020.2451 N2 - Background: MicroRNAs, as small non-coding RNAs, are recently reported to be involved in plant defense system against pathogens including fungi.Objective: In this research, it was intended to investigate candidate susceptible rice (Oryza Sativa) Osa-miRNA expression alteration following the infection by Rhizoctonia solani.Materials and Methods: To this aim, literature review suggested eight conserved plant miRNAs that are involved in other plant-pathogen interactions. Then, sixty days old rice plants (Hashemi, susceptible cultivar) were inoculated with R. solani and candidate miRNA expression alterations were investigated 2 hpi (hours post inoculation), 2 dpi (days post inoculation) and 6 dpi.Results: RT-qPCR analysis suggested four subgroups of candidate miRNAs based on the time of their responses to the pathogenesis of R. solani. While Osa-miR-156 was early-responsive, Osa-miR159 was the last-responsive and Osa-miR167, Osa-miR171, Osa-miR408, and Osa-miR444 were late responsive to R. solani infection. Osa-miR166 and Osa-miR393 were non-responsive to this infection, compared to the mock-inoculated control group. Consistently, Os-SPL3 and Os-MADS known target genes were expressed in reverse correlation to Osa-miR156 and Osa-miR444, respectively.Conclusions: From these data, it is suggested that both early (Osa-miR-156) and late (Osa-miR167, Osa-miR171, OsamiR408, Osa-miR444) responsive miRNAs might be involved in R. solani infection in rice plants. UR - https://www.ijbiotech.com/article_111374.html L1 - https://www.ijbiotech.com/article_111374_cfccb93c9389b5a1aa4ba955ff03bd5a.pdf ER -