National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Molecular Basis of Differential Gene Expression in Themouse Preimplantation Embryo
67
78
EN
HesamDehghani
Dehghani
Department of Physiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-
1973, Mashhad, I.R. Iran and Embryonic and Stem Cell Biology
dehghani@ferdowsi.um.ac.ir
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Preimplantation development of the mammalian <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">embryo consists of stages that include formation of the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">zygote, blastocyst formation and implantation of the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">embryo into the uterus. Depending on the animal, first <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">few cleavages of the early embryo is fully supported by <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">translation of maternal transcripts and use of maternal <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">proteins. After this period, the preimplantation embryo <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">starts to transcribe from its own genome and produce <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">products, which are necessary for further development. Eventually, differential gene expression results <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">in production of three cell types in the preimplantation <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">embryo; an outer transporting polarized epithelium <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(trophoblast) and two cell types of primitive endoderm <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(hypoblast), and epiblast in the inner cell mass. After <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">implantation, the trophoblast and hypoblast give rise to <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">extra-embryonic tissues and epiblast cells form primarily the embryo proper. Expression of maternal and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">embryonic transcripts and proteins, and differential <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">expression of these products that lead to differentiation <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">of embryonic cells are all highly coordinated events, <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">which need to be temporally and spatially regulated <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">during this period of development. In this review article <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">mechanisms and paradigms that may define and regulate these cellular activities leading to the first cellular <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">differentiation of life are presented. Considering the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">abundance of research data on the preimplantation <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">development of rodents, in this review we will mainly <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">focus on the mouse model.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span>
Preimplantation development,differentiation,signaling pathways,Chromatin remodeling
https://www.ijbiotech.com/article_7024.html
https://www.ijbiotech.com/article_7024_b492152e20a162a9e284e760fb8f201f.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Repair of Old Myocardial Infarction by Intracoronary Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells: A Pilot Clinical Trial
79
86
EN
Amir Farhang
Zand Parsa
Department of Cardiology, Imam Khomeini Medical Center, Keshavarz Blvd. P.O. Box 14197-33141, Tehran,
I.R. Iran
zandparsa@tums.ac.ir
Mandana
Mohyeddin Bonab
Shariati Hematology-Oncology and Bone Marrow Transplantation (BMT) Research Center,
P.O. Box 14114 Tehran, I.R. Iran
Kamran
Alimoghaddam
Shariati Hematology-Oncology and Bone Marrow Transplantation (BMT) Research Center,
P.O. Box 14114 Tehran, I.R. Iran
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">Experimental and clinical studies have shown that intracoronary transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) has resulted in regenerated infarcted myocardium and improved left ventricular (LV) function. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">The aim of this pilot study was to assess the benefical effects <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">of intracoronary transplantation of BMSC in patients with old <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">myocardial infarction (OMI). Autologous BMSCs were transplanted by the intracoronary method <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 9pt;"><em>via </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">percutaneous transluminal coronary balloon angioplasty (PTCA) in five patients <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">with old myocardial infarction. Time from myocardial infarction (MI) to cell therapy was 5.2 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">3.11 months (mean <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">SD). <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">All patients were <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;"><<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">70 years old (32-61 years) and had significant LV dysfunction (LV ejection fraction, mean <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">SD, 34<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">% <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">10.83<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">%<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">), and severe wall motion abnormality (akinesia and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">/ dyskinesia) at the location of infarcted area. Follow up <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">angiography was performed 6-9 months (mean <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">SD,7 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">1.4 <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">months) after BMSC transplantation, which revealed an <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">increased trend in the LV ejection fraction (LVEF) of patients <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">after treatment (LVEF: Mean <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">SD from 34<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">% ± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">10.83<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">% <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">to <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">46.25<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">% ± <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">9.46<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">%<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">, P= 0.051 and median from 35<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">% <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">to 42.5<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 9pt;">%<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">). <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">Clinical follow up (for 12-18 months) also revealed appreciable improvement in their symptoms or functional class [dyspnea from New York Heart Association(NYHA)-Class Ш-IV to <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">I–II and Chest discomfort from Canadian Cardiovascular <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">Society (CCS) Class II-IV to I-II]. Intracoronary transplantation of autologous BMSC in patients with old myocardial <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">infarction appears to be feasible, safe and effective .The <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">therapeutic effect could be attributed to BMSCs ability to <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 9pt;">regenerate myocardium</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Myocardial Infarction,Bone marrow mesenchymal stem cell,Percutaneous Coronary Intervention,Left ventricular function,Autologus Transplantation
https://www.ijbiotech.com/article_7023.html
https://www.ijbiotech.com/article_7023_af6389efeeef9748b120c12bb20e1ca8.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Modeling of Single Cell Protein Production from Cheese Whey Using Tanks-in-Series Model
87
92
EN
Marjan
Varedi Kolaei
Department of Biotechnology, Faculty of Science, University of Tehran, P.O. Box 1198-16765, Tehran,
I.R. Iran
Ramin
Karimzadeh
Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modarres University,
P.O. Box 14155-4843, Tehran, I.R. Iran
ramin@modares.ac.ir
Seyed Abbas
Shojaosadati
Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modarres University,
P.O. Box 14155-4843, Tehran, I.R. Iran
shoja@modares.ac.ir
Jafar
Towfighi
Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modarres University,
P.O. Box 14155-4843, Tehran, I.R. Iran
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">In this work, mathematical modeling of microbial <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(<span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Trichosporon </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">sp<span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>.</em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">) biomass production in a stirred tank <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">bioreactor and in an external airlift bioreactor has been <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">investigated. A model based on a tanks-in-series <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">model without back-flow has been used to simulate the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">production of single cell protein in the external airlift <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">bioreactor under an unsteady condition and without <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">oxygen limitation, utilizing cheese whey as a substrate. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">The kinetic parameters of cell growth and substrate <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">consumption including <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 7pt;">m<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(maximum specific growth), <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">K <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 7pt;">s<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(growth associated parameter), <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">γ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(saturated constant) and <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">λ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(non growth associated parameter) were <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">determined based on experimental data derived from <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">the batch process in the stirred tank reactor and the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">kinetic model, which resulted in 0.59 h<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 7pt;">-1<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">, 46.84 g/l, <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">0.383 and 1.275, respectively. Estimated biokinetic <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">parameters were applied to find the profiles of biomass <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and lactose in the airlift bioreactor. MATLAB software <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">was used to find kinetic parameters and solve the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">equations of the tanks-in-series model. The number of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">stages of the tanks-in-series obtained equals 16.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Modeling,Cheese whey,External airlift
bioreactor,Single cell protein,Tanks-in-series model
https://www.ijbiotech.com/article_7026.html
https://www.ijbiotech.com/article_7026_b5edc443386fb57da0ef8cf033bd38b6.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Helix Segment Assignment in Proteins Using Fuzzy Logic
93
99
EN
Shahriar
Arab
Department of Bioinformatics, Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-
1384, Tehran, I.R. Iran
Farzad
Didehvar
Institute for Studies in Theoretical Physics and Mathematics (IPM), Niavaran Square,
P.O. Box 19395-5746, Tehran, I.R. Iran
Changiz
Eslahchi
0000000000000000
Faculty of Mathematical Sciences, Shahid Beheshti University, Evin,
Tehran, I.R. Iran
ch-eslahchi@sbu.ac.ir
Mehdi
Sadeghi
Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology,
P.O. Box 14155-6343, Tehran, I.R. Iran
sadeghi@nigeb.ac.ir
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">The automatic assignment of protein secondary structure from three dimensional coordinates is an essential <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">step in the characterization of protein structure. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Although, the recognition of secondary structures such <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">as alpha-helices and beta-sheets seem straightforward, but there are many different definitions, each <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">regarding different criteria. We have developed a new <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">algorithm for protein helix assignment, by using fuzzy <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">logic based on backbone torsion angles. In this <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">method, each residue takes a number from 0 to 100 <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">that indicates the helical membership degree of that <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">residue. This method can be converted to a classical <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">method whenever we assume that any residue with a <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">membership degree greater than 83 is a helix. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Comparison of the results with structures reported in <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">protein data bank (PDB), dictionary of secondary <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">structure of proteins (DSSP) and structure identification (STRIDE) for 324 proteins indicate that our algorithm works as well as DSSP showing 93% agreement. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">We believe that the fuzzy secondary structure assignment has more advantages than the other classical <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">approaches used for protein structure comparisons <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and alignments.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span>
Protein structure,Secondary structure assignment,Fuzzy logic
https://www.ijbiotech.com/article_7007.html
https://www.ijbiotech.com/article_7007_3a01acf822ef17f9a2370bdf78741cdd.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
A Protocol for Mass Production of Rosa hybrida cv. Iceberg Through in vitro Propagation
100
104
EN
Pegah
Khosravi
Department of Crop sciences, University of Sari, Sari, I.R. Iran
Maryam
Jafarkhani Kermani
Department of Tissue Culture and Gene
Transformation, Agricultural Biotechnology Research Institute of Iran, P.O. Box 31535-1897, Karaj, I.R. Iran
m.j.kermani@abrii.ac.ir
Gorban Ali
Nematzadeh
Department of Crop sciences, University of Sari, Sari, I.R. Iran
Mohammad Reza
Bihamta
Department of Plant Breeding, University of Tehran, P.O. Box 31587-8710, Karaj, I.R. Iran
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Interactive effect of plant growth regulators <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">6-Benzylaminopurine (BAP) (0, 2, 4 and 8 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">M) and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">1-Naphtalene acetic acid (NAA) (0, 0.05, 0.25 and 0.5 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">M) in Van der Salm (VS) medium was used to optimize <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>in vitro </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">propagation of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Rosa hybrida </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">cv. Iceberg. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Shoot proliferation and number of new leaves were <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">measured as growth indicators. As the concentration <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">of BAP was raised, growth rate increased with all of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">the above NAA concentrations. However, the highest <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">number of axillary shoots and new leaves were produced with 4 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">M BAP, which was considered the optimal level. A multiplication rate of 10 folds with a maximum number of axillary shoots (10.1) and new leaves <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">per explant (25) were obtained in the medium containing 4 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">M BAP plus 0.5 <span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">µ<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">M NAA. <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>In vitro</em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">-derived shoots <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">were used to investigate root initiation and growth by <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">lowering the concentration of VS mineral salts and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">vitamins. Three strengths of VS (full, 1/2 and 1/4) were <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">compared in semi-solid and liquid medium. The average number of roots (4.35) and root length (0.82 cm) <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">were significantly higher in 1/4 strength VS. The highest percentage of rooting (93.33%) and number of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">roots (4.45) were significantly higher in semi-solid than <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">liquid medium. The regenerated plantlets were successfully transferred to soil and the survival rates of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">the rooted plantlets transferred to soil were 70% and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">90% in plants treated with semi-sold and liquid media, <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">respectively</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
In vitro Propagation,Root initiation,Rosa hybrida,Shoot proliferation
https://www.ijbiotech.com/article_7014.html
https://www.ijbiotech.com/article_7014_29f3492c705de224e21062a8ab7166f9.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Integration of A Lipase Gene into the Bacillus subtilis Chromosome: Recombinant Strains Without Antibiotic Resistance Marker
105
109
EN
Hassan
Motejadded
Institute of Industrial Genetics, Allmandring 31, University of Stuttgart, 70569 Stuttgart, Germany
hassan.motejadded@iig.uni-stuttgart.de
Josef
Altenbuchner
Institute of Industrial Genetics, Allmandring 31, University of Stuttgart, 70569 Stuttgart, Germany
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">A new system is presented for the generation of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Bacillus subtilis </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">strains without antibiotic <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">markers. This system is based on two plasmids constructed in <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Escherichia coli</em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">. The first plasmid pHM30 <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">contains an incomplete <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">gene, the last gene in the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">histidine biosynthesis operon of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>B. subtilis </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and part of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">the genes <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>yvcA </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>yvcB </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">of unkown function flanking <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">at the 3´-end. The spectinomycin resistance gene <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">is inserted between <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and the downstream <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>yvcAB</em> <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">region. Transformation of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>B. subtilis </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">with this plasmid <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">pHM30 led to spectinomycin resistant, histidine auxotrophic strains. The integrated parts of pHM30 act like <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">a docking station for the second plasmid pHM31. The <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">plasmid pHM31 contains the same <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>yvcAB </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">region but a <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">complete copy of the <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">gene and no antibiotic resistance marker. Heterologous genes to be expressed in <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>B. subtilis </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">were inserted into a multiple cloning site <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">between <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and the downstream region. <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Transformants of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>B.subtilis</em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">/pHM30 with pHM31 derivatives were selected on minimal medium without histidine. By double crossovers during homologous recombination the heterologous genes were integrated, <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">replacing the defect copy of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>hisI </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and the spectinomycin <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">resistance gene. The plasmids were also successfully <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">applied in the chromosomal integration of the lipase <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">gene of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Bacillus thermocatenulatus </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">under a <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>B. subtilis</em> <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">glucose regulated promotor/antiterminator system.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Chromosomal integration,Antibiotic marker free strains,Food-grade organisms
https://www.ijbiotech.com/article_7029.html
https://www.ijbiotech.com/article_7029_8b893d8a6749240f50366d7fd6527202.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
110
113
EN
Hamidreza
Falahatpishe
Department of Food Technology Research, National Nutrition and Food Technology Research Institute,
Shahid Beheshti University, M.C., P.O. Box 19395-4147, Tehran, I.R. Iran
hfalahatpishe@yahoo.com
Mahmoud
Jalali
Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran
Naser
Badami
Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran
Nadia
Mardani
Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran
Kianoush
Khosravi-Darani
Department of Food Technology Research, National Nutrition and Food Technology Research Institute,
Shahid Beheshti University, M.C., P.O. Box 19395-4147, Tehran, I.R. Iran
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">This research has focused on isolation and characterization of a strain of <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Bacillus </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">sp<span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>. </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">from alkaline soil, <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">which was able to produce extracellular alkaline protease at pH<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 7pt;">s <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">ranging from 8 to 11 and temperatures of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">20 to 50<span style="font-variant: normal; font-style: normal; font-family: TimesNewRomanPSMT; color: #231f20; font-size: 10pt;">ºC<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">. Also the impact of different carbon and <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">nitrogen sources were investigated. The yield and fold <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">of enzyme purification was 24<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">% <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Bacillus </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">sp. 2 - 5 showed the most <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">caseinolytic activity (without any gelatinolytic activity) <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">at pH<span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;">><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">10</span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Alkalophilic Bacillus,Protease,Process variables,Purification
https://www.ijbiotech.com/article_9426.html
https://www.ijbiotech.com/article_9426_f4e408bdf8aee17bd08445f937d29917.pdf
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
5
2
2007
04
01
Biosorption of Textile Dyes and Effluents by Pleurotusflorida and Trametes Hirsutawith Evaluation of Their Laccase Activity
114
118
EN
Sathiya Moorthi
Perumal
Department of Industrial Biotechnology, Dr. M.G.R. Educational and Research Institute, Dr. M.G.R. University,
Chennai-600 095, India
sathiya.india@gmail.com
Deecaraman
Munuswamy
Department of Industrial Biotechnology, Dr. M.G.R. Educational and Research Institute, Dr. M.G.R. University,
Chennai-600 095, India
Periyar Selvam
Sellamuthu
Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India
Murugesan
Kandasamy
Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India
Kalaichelvan
Puthupalayam Thangavelu
Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India
<span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 7pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: TimesNewRomanPSMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: SymbolMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;"><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">The rate and efficiency of decolorization of dyes like <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">Blue CA, Black B133, and Corazol Violet SR were tested to evaluate white rot fungal strains. <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Trametes hirsuta </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">and <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Pleurotus florida </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">showed the greatest extent of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">decolorization on nutrient salt media. Maximum decolorization of 200 mg/l of Blue 133 was obtained by 4 <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">days old incubated <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Pleurotus florida </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">followed by <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>Trametes hirsuta </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">after 6 days<span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>. </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">An attempt was made to <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">improve the decolorization activity of both organisms <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">with different concentrations of glucose 1 and 2% <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">(w/v). The decolorization activity may be due to the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">laccase enzyme of white rot fungi. The production of <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">this enzyme was estimated using solid state fermentation with rice bran as a substrate. It was found that <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>P.</em> <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>florida </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">exhibited 0.175 U/ml of laccase activity followed <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">0.126U/ml by <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>T. hirsute</em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">, respectively. Decolourization <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">was found to be more effective with <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt;"><em>P. florida </em><span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">in the <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">presence of 2% (w/v) glucose. Crude extract containing the laccase enzyme was isolated and confirmed by <span style="font-variant: normal; font-style: normal; font-family: ArialMT; color: #231f20; font-size: 10pt;">SDS PAGE</span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="line-height: normal; widows: 2; text-transform: none; font-variant: normal; font-style: normal; text-indent: 0px; white-space: normal; orphans: 2; letter-spacing: normal; font-weight: normal; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Dye house effluent,Laccase,Pleurotus florida,reactive textile dyes,Trametes hirsuta
https://www.ijbiotech.com/article_7030.html
https://www.ijbiotech.com/article_7030_668dfcef961b0e1f6af7f806ec70d6e5.pdf