ORIGINAL_ARTICLE
Expression of Regulated Oncogen-Alpha by Primary Hepatocytes Following Isolation and Heat Shock Stimulation
High levels of regulated oncogen-alpha (GRO-a) expression have been observed in the liver. GRO-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. Given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. In this study, hepatocytes (2×106 cell/ml) were isolated from male Sprague Dawley rat liver and cultured on plates that were pre-coated with collagen type-I matrix. The western and northern blot analyses were employed to detect GRO-a at the protein and mRNA levels in freshly isolated and cultured hepatocytes in response to isolation and heat shock stresses. GRO-a was shown to be expressed by isolated rat hepatocytes immediately after isolation and early culture and decreased with time. mRNA was also expressed in freshly isolated cells (0 h) and did not decrease after 48h of culture and further time points (P<0.01). These results also demonstrated that expression of GRO-a by hepatocytes increased in response to heat shock at different time points in comparison with the control (P<0.01). These results demonstrated that the isolation and heat shock stresses induced the expression of GRO-a in hepatocytes in a time-dependent manner. Thus, it seems that hepatocytes mimic the experiences that the liver encounters after injury in vivo. In such a situation, liver produces stress related agents like chemokines to overcome injurious conditions.
https://www.ijbiotech.com/article_7090_45a954368f86f0f00bbf5abdc7730b43.pdf
2009-01-01
1
9
Hepatocyte
GRO-a
Chemokine
Heat shock
Azim
Mousavizadeh
1
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Mohammadreza
Mohammad Abadi
mmohammadabadii@yahoo.ca
2
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
LEAD_AUTHOR
Azam
Torabi
3
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
AUTHOR
Mohammad Reza
Nassiry
4
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Heydar
Ghiasi
5
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Ali
Esmailizadeh Koshkoieh
6
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
AUTHOR
Baggiolini M, Dewald B, Moser B (1997). Human chemokines: an update. Annu Rev Immunol. 15: 675-705.
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Diehl AM (2000). Cytokine regulation of liver injury and repair. Immunol Rev. 174: 160-171.
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Haskill S, Peace A, Morris J, Sporn SA, Anisowicz A, Lee SW, Smith T, Martin G, Ralph P, Sager R (1990). Identification of three related human GRO genes encoding cytokine functions. Proc Natl Acad Sci USA. 87: 7732-7736.
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Hassanshahi G, Kazemi arababadi M, Dickson James A (2006). Expression of CXC Chemokines GRO/KC and SDF-1 alfa in Rat H4 Hepatoma Cells in Response to Different Stimuli. Iran J Immunol. 3: 54-60.
4
Hassanshahi G, Jafarzadeh A, James Dickson A (2008). Expression of stromal derived factor alpha (SDF-1 alpha) by primary hepatocytes following isolation and heat shock stimulation. Iran J Allergy Asthma Immunol. 7: 61-68.
5
Hassanshahi G, Patel SS, Jafarzadeh AA, Dickson AJ (2007a). Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock. Saudi Med. J 28: 514-518.
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Hassanshahi G, Jafarzadeh A, Ghorashi Z, Zia Sheikholeslami N, Dickson AJ (2007b). Expression of IP-10 chemokine is regulated by pro-inflammatory cytokines in cultured hepatocytes. Iran J Allergy Asthma Immunol. 6: 115-121.
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Maroni P, Bendinelli P, Zuccorononno C, Schiaffonati L, Piccoletti R (2000). Cellular signalling after in vivo heat shock in the liver. Cell Biol Int. 24: 145-152.
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Mercer-Jones MA, Shrotri MS, Peyton JC, Remick DG, Cheadle WG (1999). Neutrophil sequestration in liver and lung is differentially regulated by C-X-C chemokines during experimental peritonitis. Inflammation 23: 305-319.
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17
Olszyna DP, Pajkrt D, van Deventer SJ, van der Poll T (2001). Effect of interleukin 10 on the release of the CXC chemokines growth related oncogene GRO-alpha and epithelial cell-derived neutrophil activating peptide (ENA)-78 during human endotoxemia. Immunol Lett. 78: 41-44.
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Shiratori Y, Hikiba Y, Mawet E, Niwa Y, Matsumura M, Kato N, Shiina S, Tada M, Komatsu Y, Kawabe T, et al. (1994). Modulation of KC/GRO protein (interleukin-8 related protein in rodents) release from hepatocytes by biologically active mediators. Biochem Biophys Res Commun. 203: 1398-1403.
22
Su AI, Guidotti LG, Pezacki JP, Chisari FV, Schultz PG (2002). Gene expression during the priming phase of liver regeneration after partial hepatectomy in mice. Proc Natl Acad Sci USA. 99: 11181-11186.
23
Suzuki H, Mori M, Seto K, Shibata F, Nagahashi S, Kawaguchi C, Suzuki M, Matsui H, Watanabe K, Miura S, Ishii H (2000). Rat CXC chemokine GRO/CINC-1 paradoxically stimulates the growth of gastric epithelial cells. Aliment Pharmacol. 1: 94-100.
24
Varley CL, Armitage S, Hassanshahiraviz G, Dickson AJ (2003). Regulation of the C-X-C chemokine, mob-1, gene expression in primary rat hepatocytes. Cytokine 23: 64-75.
25
Wang D, Richmond A (2001). Nuclear factor-kappa B activation by the CXC chemokine melanoma growth-stimulatory activity/growth-regulated protein involves the MEKK1/p38 mitogen-activated protein kinase pathway. J Biol Chem. 276: 3650-3659.
26
Xiong HQ, Abbruzzese JL, Lin E, Wang L, Zheng L, Xie K (2004). NF-kappaB activity blockade impairs the angiogenic potential of human pancreatic cancer cells. Int J Cancer. 108: 181-188.
27
Zhang L, Pelech S, Uitto VJ (2004). Long-term effect of heat shock protein 60 from Actinobacillus actinomycetemcomitans on epithelial cell viability and mitogen-activated protein kinases. Infect Immun. 72: 38-45.
28
Zhang XW, Liu Q, Wang Y, Thorlacius H (2001). CXC chemokines, MIP-2 and KC, induce P-selectin-dependent neutrophil rolling and extravascular migration in vivo. Br J Pharmacol. 133: 413-421.
29
ORIGINAL_ARTICLE
Production of L-Asparaginase by Serratia marcescens SB08: Optimization by Response Surface Methodology
This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the optimum values of the selected parameters using central composite design was performed. Sucrose, peptone, KH2PO4 and incubation time were found to be the best medium factors for the optimization of L-asparaginase production and central composite design experiments indicated the optimal concentrations of sucrose 12.50 g/l, peptone 4.5 g/l, KH2PO4 4.0 g/l and incubation time 51h. The combined optimization method described here is the effective for screening medium factors as well as determining their optimum levels for the production of L-asparaginase by Serratia marcescens SB08.
https://www.ijbiotech.com/article_7091_6f6ff773c02c3bb9289f1890248123e7.pdf
2009-01-01
10
18
Serratia marcescens SB08
L-asparaginase
PBD
CCD
Chidambaram
Venil
ckvenil@gmail.com
1
Department of Environmental Sciences, Division of Environmental Microbiology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
LEAD_AUTHOR
Kuppanan
Nanthakumar
2
Department of Environmental Sciences, Division of Environmental Microbiology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
AUTHOR
Kannan
Karthikeyan
3
Department of Environmental Sciences, Division of Environmental Microbiology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
AUTHOR
Perumalsamy
Lakshmanaperumalsamy
4
Department of Environmental Sciences, Division of Environmental Microbiology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
AUTHOR
Abdel-Fattah YR, Saeed HM, Gohar YM, El-Baz MA (2005). Improved production of Pseudomonas aeruginosa uricase by optimization of process parameters through statistical experimental designs. Process Biochem. 40: 1707-1714.
1
Adamson RH, Fabro S (1968). Antitumor activity and other biologic properties of L-asparaginase. Cancer Chemothe Rep. 52: 617-626.
2
Aguilar G, Morlon-Guyot J, Trejo-Aguilar B, Guyot JP (2000). Purification and characterization of an extra cellular alpha amylase produced by Lactobacillus manihotivorans LMG 1801 (T), an amylolytique lactic acid bacterium. Enzyme Microb Technol. 27: 406-413.
3
Azambuja P, Feder D, Garcia ES (2004). Isolation of Serratia marcescens in the midgut of Rhodnius prolixus: impact on the establishment of the parasite Trypanosoma cruzi in the vector. Exp Parasitol. 107: 89-96.
4
Box GEP, Hunter JS (1957). Multi-factor experimental designs for exploring response surfaces. Ann Math Stat. 28: 195- 241.
5
Box GEP, Wilson KB (1951). On the experimetal designs for exploring response surfaces. Ann Math Stat. 13: 1-45.
6
Buchanan RE, Gibbons NE (1974). Bergey’s manual of determinative bacteriology. (Eighth edition), The Williams and Wilkins Co., Baltimore. PP. 747-842.
7
Doull JL, Vining LC (1989). Culture conditions promoting dispersed growth and biphasic production of actinorhodin in shaken cultures of Streptomyces coelicolor A3(2). FEMS Microbiol Lett. 65: 265-268.
8
Kim HO, Lim JM, Joo JH, Kim SW, Hwang HJ, Choi JW, Yun JW (2005). Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Bioresour Technol. 96: 1175-1182.
9
Le Mense EH, Julian C, Van Lanen JM, Langlykke AF (1947). Production of mold amylases in submerged culture. Biochemistry. 54: 149-159.
10
Lee KM, Gilmore DF (2005). Formulation and process modeling of biopolymer (polyhydroxyalkanoates: PHAs) production from industrial wastes by novel crossed experimental design. Process Biochem. 40: 229-246.
11
Manna S, Sinha A, Sadhukhan R, Chakrabarty SL (1995). Purification, characterization and antitumor activity of L-asparaginase isolated from Pseudomonas stutzeri MB-405. Curr Microbiol. 30: 291-298.
12
Mctigue MA, Kelly CT, Fogarty WM, Doyle EM (1994). Production studies on the alkaline amylases of three alkalophilic Bacillus spp. Biotechnology Lett. 16: 569-574.
13
Nawani NN, Kapadnis BP (2005). Optimization of chitinase production using statistics based experimental designs. Process Biochem. 40: 651-660.
14
Oettgen HF, Old LJ, Boyae EA, Campbell HA, Philips FS, Clarkson, BD, Tallal L, Leper RD, Schwartz MK, Kim JH (1967). Inhibition of leukemias in man by L-asparaginase. Cancer Res. 27: 2619-2631.
15
Plackett RL, Burman JP (1946). The design of optimum multifactorial experiments. Biometrika 33: 305-325.
16
Ramesh MV, Lonsane BK (1987). A novel bacterial thermostable alpha amylase system produced under solid-state fermentation. Biotechnol Lett. 9: 501-504.
17
Ray RR (2001). Production of alpha-amylase xylanase by an alkalophilic strain of Penicillium griseoroseum RR-99. Acta Microbiol Pol. 50: 305-309.
18
Santamaria RI, Del Rio G, Saab G, Rodriguez ME, Soberon X, Lopez-Marguia A (1999). Alcoholysis reactions from starch with alpha-amylases. FEBS Lett. 452: 346-350.
19
Senthilkumar SR, Ashokkumar B, Raj K, Chandra, Gunasekaran P (2005). Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design. Bioresour Technol. 96: 1380-1386.
20
Swain AI, Jaskolski M, Housset D, Mohana Rao JK, Wlodawer A (1993). Crystal structure of Escherichia coli L-asparaginase, an enzyme used in cancer therapy. Proc Natl Acad Sci USA. 90: 1474-1478.
21
Tani S, Kawaguchi T, Kato M, Kobayashi T, Tsukagoshi N (2000). A novel nuclear factor, SREB, binds to a cis-acting element, SER, required for inducible expression of the Aspergillus oryzae taka-amylase A gene in A. nidulans. Mol General Gene. 263: 232-238.
22
Umesh K, Shamsher S, Wamik A (2007). Pharmacological and clinical evaluation of L-asparaginase in the treatment of Leukemia. Crit rev oncol hematol. 61: 208-221.
23
Wang YX, Lu ZX (2005). Optimization of processing parameters for the mycelial growth and extracellular polysaccharide production by Boletus spp. ACCC 50328. Process Biochem. 40: 1043-1051.
24
Wriston JC, Yellin TO (1973). L-asparaginase: a review. Adv Enzymol. 39: 185-248.
25
ORIGINAL_ARTICLE
Biological Phosphorus and Nitrogen Removal from Wastewater Using Moving Bed Biofilm Process
In this research, an experimental study to evaluate nutrient removal from synthetic wastewater by a lab-scale moving bed biofilm process was investigated. Also, kinetic analysis of the process with regard to phosphorus and nitrogen removal was studied with different mathematical models. For nutrient removal, the moving bed biofilm process was applied in series with anaerobic, anoxic and aerobic units in four separate reactors that were operated continuously at different loading rates of phosphorus and nitrogen and different hydraulic retention times. Under optimum conditions, almost complete nitrification with an average ammonium removal efficiency of 99.72% occurred in the aerobic reactor. In the aerobic reactor, the average specific nitrification rate was 1.92 g NOx-N (NOx-N=NO2-N +NO3-N) produced/kg volatile suspended solids. hour (VSS.h). Denitrification rate increased with increasing NOx-N loading in the second anoxic reactor. The aerobic phosphate removal rate showed good correlation with the anaerobic phosphate release rate. Under optimum conditions, the average total nitrogen and phosphorus removal efficiencies were 80.9% and 95.8%, respectively. As a result of the moving bed biofilm process (MBBR) kinetic analysis, the Stover-Kincannon model was chosen for modeling studies and experimental data analysis. The Stover-Kincannon model gave high correlation coefficients for phosphorus and nitrogen removal, which were 0.9862 and 0.986, respectively. Therefore, this model could be used in predicting the behavior or design of the moving bed biofilm process.
https://www.ijbiotech.com/article_7093_cbc84da331888d075643c4c3c9d424f9.pdf
2009-01-01
19
27
MBBR
Biofilm carriers
Biological nutrient removal (BNR)
Sewage Treatment
Stover-Kincannon model
Majid
Kermani
majidkermani@yahoo.com
1
Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, P.O. Box 15875-4199, Tehran, I.R. Iran
LEAD_AUTHOR
Bijan
Bina
2
Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan, I.R. Iran
AUTHOR
Hossein
Movahedian
3
Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan, I.R. Iran
AUTHOR
Mohammad Mehdi
Amin
4
Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan, I.R. Iran
AUTHOR
Mahnaz
Nikaeen
5
Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan, I.R. Iran
AUTHOR
Andreottola G, Foladori P, Ragazzi M, Tatano F (2000a). Experimental comparison between MBBR and activated sludge system for the treatment of municipal wastewater. Water Sci Technol. 41: 375-382.
1
Andreottola G, Foladori P, Ragazzi M (2000b). Upgrading of a small wastewater treatment plant in a cold climate region using a moving bed biofilm reactor (MBBR) system. Water Sci Technol. 41: 177-185.
2
Andreottola G, Foladori P, Ragazzi M, Villa R (2002). Dairy wastewater treatment in a moving bed biofilm reactor. Water Sci Technol. 45: 321-328.
3
Andreottola G, Foladori P, Gatti G, Nardelli P, Pettena M, Ragazzi M (2003). Upgrading of a Small Overloaded Activated Sludge Plant Using a MBBR System. J Environ Sci Health. Part A. A 38: 2317-2328.
4
APHA, AWWA, WEF (1998). Standard methods for the examination of water and wastewater. 20th Eds., Washington DC., USA.
5
Borghei SM, Sharbatmaleki M, Pourrezaei P, Borghei (2008). Kinetics of organic removal in fixed-bed aerobic biological reactor. Bioresource Technol. 99: 1118-1124.
6
Chen S, Sun D, Chung JS (2008). Simultaneous removal of COD and ammonium from landfill leachate using an anaerobic–aerobic moving-bed biofilm reactor system. Waste Manage. 28: 339-346.
7
Chuang SH, Ouyang CF, Yuang HC, You SJ (1998). Evaluation of phosphorus removal in anaerobic-anoxic-aerobic system-via polyhydroxyalkonoates measurements. Water Sci Technol. 38: 107-114
8
Dulkadiroglu H, Cokgor EU, Artan N, Orhon D (2005). The effect of temperature and sludge age on COD removal and nitrification in a moving bed sequencing batch biofilm reactor. Water Sci Technol. 51: 95-103.
9
Helness H (2007). Biological phosphorous removal in a moving bed biofilm reactor. Doctoral Dissertation, Norwegian University of Science and Technology, Norway.
10
Hem LJ, Rusten B, Ødegaard H (1994). Nitrification in a moving bed biofilm reactor. Water Sci Technol. 28: 1425-1433.
11
Hooshyari B, Azimi A, Mehrdadi N (2009). Kinetic analysis of enhanced biological phosphorus removal in a hybrid integrated fixed film activated sludge process. Int J Environ Sci Tech. 6: 149-158.
12
Hosseini SH, Borghei SM (2005). The treatment of phenolic wastewater using a moving bed bio-reactor. Process Biochem. 40: 1027-1031.
13
Hosseiny SH, Borghei SM (2002). Modelling of organic removal in a moving bed biofilm reactor (MBBR). Scientica Iranica. 9: 53-58.
14
Jahren SJ, Rintala JA, Ødegaard H (2002). Aerobic moving bed biofilm reactor treating thermomechanical pulping whitewater under thermophilic conditions. Water Res. 36: 1067-1075.
15
Johnson CH, Page MW, Blaha L (2000). Full scale moving bed biofilm reactor results from refinery and slaughter house treatment facilities. Water Sci Technol. 41: 401-407.
16
Kishida N, Kim J, Tsuneda S, Sudo R (2006). Anaerbic/oxic/anoxic granular sludge process as an effective nutrient removal process utilizing denitrifying polyphosphate-accumulating organisms. Water Res. 40: 2303-2310.
17
Luostarinen S, Luste S, Valentin L, Rintala J (2006). Nitrogen removal from on-site treated anaerobic effluents using intermittently aerated moving bed biofilm reactors at low temperatures. Water Res. 40: 1607-1615.
18
Ødegaard H, Rusten B, Westrum T (1994). A new moving bed biofilm reactor-applications and results. Water Sci Technol. 29: 157-165.
19
Ødegaard H, Rusten B, Siljudalen J (1999). The development of the moving bed biofilm process-from idea to commercial product. Eur Wat Manage. 2: 36-43.
20
Ødegaard H (2006). Innovations in wastewater treatment: the moving bed biofilm process. Water Sci Technol. 53: 17-33.
21
Okunuki S, Kawaharasaki M, Tanaka H, Kanagawa T (2004). Changes in phosphorus removing performance and bacterial community structure in an enhanced biological phosphorus removal reactor. Water Res. 38: 2433-2439.
22
Pastorelli G, Andreottola G, Canziani R, Darriulat C, de Fraja Frangipane E, Rozzi A (1997a). Organic carbon and nitrogen removal in moving-bed biofilm reactors. Water Sci Technol. 35: 91-99.
23
Pastorelli G, Andreottola G, Canziani R, de Fraja Frangipane E, de Pascalis F, Gurrieri G, Rozzi A (1997b). Pilot-plant experiments with moving bed biofilm reactors. Water Sci Technol. 36: 43-50.
24
Pastorelli G, Canziani R, Pedrazzi L, Rozzi A (1999). Phosphorus and nitrogen removal in moving-bed sequencing batch biofilm reactors. Water Sci Technol. 40: 169-176.
25
Rusten B, Ødegaard H, Lundar A (1992). Treatment of dairy wastewater in a novel moving bed biofilm reactor. Water Sci Technol. 26: 703-711.
26
Rusten B, Siljudalen JG, Nordeidet B (1994). Upgrading to nitrogen removal with the KMT moving bed biofilm process. Water Sci Technol. 29: 185-195.
27
Rusten B, Hem L, Ødegaard H (1995a). Nitrification of municipal wastewater in moving-bed biofilm reactors. Water Environ Res. 67: 75-86.
28
Rusten B, Hem L, Ødegaard H (1995b). Nitrogen removal from dilute wastewater in cold climate using moving bed biofilm reactors. Water Environ Res. 67: 65-74.
29
Rusten B, Siljudalen JG, Strand H (1996). Upgrading of a biological-chemical treatment plant for cheese factory wastewater. Water Sci Technol. 34: 41-49.
30
Rusten B, Kolkinn O, Ødegaard H (1997). Moving bed biofilm reactors and chemical precipitation for high efficiency treatment of wastewater from small communities. Water Sci Technol. 35: 71-79.
31
Rusten B, Siljudalen JG, Wien A, Eidem D (1998). Biological pretreatment of poultry processing wastewater. Water Sci Technol. 38: 19-28.
32
Rusten B, Eikebrokk B, Ulgenes Y, Lygren E (2006). Design and operations of the Kaldnes moving bed biofilm reactors. Aquacult Eng. 34: 322-331.
33
Sedlak R (1991). Phosphorus and Nitrogen Removal from Municipal Wastewater: Principles and Practice. 2nd Eds., Lewis Publishers, New York.
34
Tchobanoglous G, Burton FL, Stensel HD (2003). Wastewater engineering: treatment and reuse. 4th Eds., McGraw Hill, New York.
35
Wang XJ, Xia SQ, Chen L, Zhao JF, Renault NJ, Chovelon JM (2006). Nutrients removal from municipal wastewater by chemical precipitation in a moving bed biofilm reactor. Process Biochem. 41: 824-828.
36
ORIGINAL_ARTICLE
Linkage Map Construction for Silkworm (Bombyx mori L.) Based on Amplified Fragment Length Polymorphism Markers
The domesticated silkworm, Bombyx mori, is of high commercial importance as a silk producer and is also widely used for implementation of basic and applied research. It is important to understand its genome organization using molecular markers for genetic studies and for breeding purposes. In this study, a genetic linkage map using 204 amplified fragment length polymorphism (AFLP) markers was developed. Twenty PstI/TaqI primer combinations were used to genotype 78 progenies from an F2 population of the P107×Khorasan Lemon cross. Each primer combination generated an average of 10.2 AFLP markers qualified for linkage mapping. All the 204 AFLP markers were assigned to 12 linkage groups at the Logarithm of Odds (LOD) threshold of 2. The number of markers in the linkage groups ranged from 2 to 53. There were seven major linkage groups with 13-53 markers and five small linkage groups with 2-6 markers. The 12 linkage groups varied in length from 12.3 to 938.4 cM and the total length of linkage map was 4262 cM, giving an average marker resolution of 20.89 cM. This study presents the preliminary step for further marker-assisted research on silkworm, including Quantitative Trait Loci (QTL) and introgression analyses.
https://www.ijbiotech.com/article_7074_62bc1546a6fc4bc4edba0ff299af8b1f.pdf
2009-01-01
28
36
Silkworm
Bombyx mori
Linkage map
AFLP Markers
Seyed Ziyaeddin
Mirhoseini
1
Department of Animal Sciences, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht, I.R. Iran
AUTHOR
Babak
Rabiei
2
Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht, I.R. Iran
AUTHOR
Payam
Potki
3
Department of Genomics, Agricultural Biotechnology Research Institute of North Region (Rasht), P.O. Box 41635-4115, Rasht, I.R. Iran
AUTHOR
Seyed Benyamin
Dalirsefat
bendalir@guilan.ac.ir
4
Department of Sericulture, Faculty of Agricultural Sciences, University of Guilan, P.O. Box 41635-1314, Rasht, I.R. Iran
LEAD_AUTHOR
Bai G, Tefera H, Ayele M, Nguyen HT (1999). A genetic linkage map of tef (Eragrostis tef (Zucc.) Trotter) based on amplified fragment length polymorphism. Theor Appl Genet. 99: 599-604.
1
Becker J, Vos P, Kuiper M, Salamini F, Heun M (1995). Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet. 249: 65-73.
2
Castiglioni P, Pozzi C, Heun M, Terzi V, Mu¨ller KJ, Rohde W, Salamini F (1998). An AFLP-Based Procedure for the Efficient Mapping of Mutations and DNA Probes in Barley. Genetics 149: 2039-2056.
3
Causse MA, Fulton TM, Cho YG, Ahn SN, Chumwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Hanington SE, Second G, McCouch SR, Tanksley SD (1994). Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251-1274.
4
Dalirsefat SB, Mirhoseini SZ (2007). Assessing Genetic diversity in Iranian native silkworm (Bombyx mori L.) strains and Japanese commercial lines using AFLP markers. Iranian J Biotechnol. 5: 25-33.
5
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ORIGINAL_ARTICLE
Molecular Assessment of Clonal Fidelity in Micropropagated Grape (Vitis spp.) Rootstock Genotypes Using RAPD and ISSR Markers
Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vitro derived plantlets. A dozen RAPD (10-mer) and ten ISSR (dinucleotide contained repeats) primers were used for PCR and reproducible band profiles were obtained. The 84 and 81 distinct and scorable band classes (a total of 1,914 and 1,980 scorable bands) with an average of 7.0 and 8.1 bands per primer were obtained by RAPD and ISSR, respectively. Although higher numbers of bands were obtained by ISSR rather than RAPD, but none of the primers showed polymorphism among micropropagated plantlets and their respective mother plants. The profiles generated based on the two marker systems were found to be highly uniform and monomorphic. Cluster analysis further confirmed genetic stability of micropropagated plantlets. Jaccard’s similarity coefficients obtained for both markers in mother plants and their in vitro regenerants were estimated to be 1.00 but three sets of genotypes were grouped into two major clusters with similarity coefficients of 0.53 (RAPD) and 0.63 (ISSR). The molecular analyses precisely proved the production of genetically stable grape plantlets and certified the application of micropropagation protocol to be developed on a commercial scale.
https://www.ijbiotech.com/article_7094_c6da289d33b96c24485ddc73a13edf98.pdf
2009-01-01
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Vitis rootstocks
Micropropagation
Clonal fidelity
RAPD
ISSR
Mahdi
Alizadeh
guasnr@gmail.com
1
Department of Horticulture, Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), P.O. Box 386, Gorgan, I.R. Iran
LEAD_AUTHOR
Sanjay
Singh
2
Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute (IARI), New Delhi 110 012, India
AUTHOR
Alizadeh M, Singh SK, Tripta Jhang, Sharma TR (2008). Inter simple sequence repeat analysis to confirm genetic stability of micropropagated plantlets in three grape (Vitis spp.) rootstock genotypes. J Plant Biochem Biotech. 17: 77-80.
1
Alizadeh M (2007). Micropropagation and in vitro screening of some grape (Vitis spp.) rootstock genotypes for salt tolerance. Ph.D. thesis submitted to the P.G.School, Indian Agricultural Research Institute, New Delhi, India.
2
Boxus PH, Jemmali A, Terzi JM, Arezki O (2000). Drift in genetic stability in micropropagation: The case of strawberry. Acta Hort. 530: 155-162.
3
Carvalho LC, Goulao L, Oliveira C, Goncalves JC, Amancio S (2004). RAPD assessment for identification of clonal fidelity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tiss Org Cult. 77: 23-27.
4
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5
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6
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12
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32
ORIGINAL_ARTICLE
Evaluation of Stability of Chitinase Gene in Transgenic Offspring of Cotton (Gossypium hirsutum)
Cotton cultivar Coker has been already transformed with recombinant pBI121-chi via Agrobacterium tumefaciens. The T-DNA region of pBI121-chi carries the chitinase (chi ) gene from bean and is under the control of the CaMV35S promoter. T1 and T2 progenies of transgenic cotton containing the chi gene were used in this study. Polymerase chain reaction (PCR), Southern and Western blotting data confirmed integration and expression of the chi gene in the T1 and T2 progenies. The growth of Verticillium dahliae was singnificantly inhibited in an in vitro bioassay for which 100 μg of crude leaf protein extract derived from the T1 plants was used. The 850-bp expected chi fragment was amplified for 77 transgenic plants from 128 T1 and T2 progenies, and 75 transgenic plants showed both chi and nptII bands. T0 conduct bioassay, cotton seedlings were infected with the spore suspension (106 spores/ml), in a greenhouse. Fifty-five percent of the transgenic plants were able to restrict V. dahliae growth and symptoms. There were no distinguishable differences in the phenotypic appearance of transgenic plants compared to non-transgenics. These results showed that transgenic cotton expressing a bean chitinase exhibited enhanced resistance against V. dahliae in greenhouse and in-vitro assay as compared to the non-transgenic plants.
https://www.ijbiotech.com/article_7073_7c675b49ec4faa3ddd1fed830cfc53e5.pdf
2009-01-01
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50
Chitinase gene
tolerance
Transgenic cotton
Verticillium dahliae
Masoud
Tohidfa
gtohidfar@yahoo.com
1
Agricultural Biotechnology Research Institute of Iran (ABRII) P.O. Box 31535-1897, karaj, I.R. Iran
LEAD_AUTHOR
Hassan
Rassouli
2
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, P.O. Box 313, Zanjan, I.R. Iran
AUTHOR
Behzad
Ghareyazie
3
Agricultural Biotechnology Research Institute of Iran (ABRII) P.O. Box 31535-1897, karaj, I.R. Iran
AUTHOR
Javad
Najafi
4
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, P.O. Box 313, Zanjan, I.R. Iran
AUTHOR
Adams DJ (2004). Fungal cell wall chitinase and glucanases. Microbiology 150: 2029-2035.
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ORIGINAL_ARTICLE
Genetic Polymorphism at the Growth Hormone Locus in Iranian Talli Goats by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP)
The growth hormone gene could be an attractive candidate gene for milk production in goats. Single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone (gGH) gene. For this purpose, genotyping of 90 Talli goat breeds was performed. Nine conformational patterns were observed in exon 4 of the gGH gene, with frequencies of 27.7% for the homozygous pattern (AA) and 72.2% for all of other heterozygous patterns (A/B, A/C, A/B/C, A/B/D/E, A/B/C/F, A/C/F, A/B/E, A/B/F). The results showed that exon 4 of the GH gene in Talli goats is highly polymorphic.
https://www.ijbiotech.com/article_7064_794cd2b52ee094dbde40373e59a4db4d.pdf
2009-01-01
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Growth hormone gene
Polymorphism
goat-PCR-SSCP
Azim
Mousavizadeh
1
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Mohammadreza
Mohammad Abadi
mmohammadabadii@yahoo.ca
2
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
LEAD_AUTHOR
Azam
Torabi
3
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
AUTHOR
Mohammad Reza
Nassiry
4
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Heydar
Ghiasi
5
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 917751163, Mashhad, I.R. Iran
AUTHOR
Ali
AliEsmailizadeh Koshkoieh
6
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, I.R. Iran
AUTHOR
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