%0 Journal Article %T Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Fayaz, Shima %A Fard-Esfahani, Pezhman %A Khaghani, Shahnaz %D 2013 %\ 04/01/2013 %V 11 %N 2 %P 104-108 %! Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene %K HRM %K XRCC2 %K Genotyping %R 10.5812/ijb.11450 %X Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Results: Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. Conclusions: We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene. %U https://www.ijbiotech.com/article_7223_ec268ed2b6d930c6bfe03d9fa7e664c2.pdf