%0 Journal Article %T Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Shahinsaz, Leila %A Sabahi, Farzaneh %A Karimi, Mohsen %A Behzadian, Farida %A Alavian, Seyed Moayed %A Zand, Vahid %D 2006 %\ 07/01/2006 %V 4 %N 3 %P 174-179 %! Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR %K Hepatitis Delta virus %K RT-PCR %K phylogenetic tree %K sequencing %K Iran %R %X Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen (HDAg) C-termini were used for genotyping. The results showed that 63.6% (23 of 36) of (HDAb) positive serum samples (as determined by ELISA) were also positive for HDV-RNA. Sequencing and phylogenic analysis of three Iranian HDV isolates revealed the most homology (93%) with an Italian isolate indicating a close relationship and probably a common origin for these isolates. %U https://www.ijbiotech.com/article_6976_eb77294d2658b69d0d7cb4bd55115ca7.pdf