%0 Journal Article %T Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Niavarani, Ahmadreza %A Zeinali, Sirous %A Karimi, Mohsen %A Rassoulzadegan, Minoo %D 2004 %\ 07/01/2004 %V 2 %N 3 %P 158-163 %! Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene %K transgenic mice %K Milk %K Calcitonin %K Beta-lactoglobulin %R %X Expression of foreign proteins in mammalian milk is becoming a widespread strategy for high-level productionof recombinant pharmaceuticals, especially those with the most complex post-translational modifications.A gene construct was generated, consisting of 10.7 kbp of the ovine beta-lactoglobulin (oBLG) geneincluding its promoter and 3ยด flanking region with the calcitonin coding sequences inserted in-frame into theoBLG fifth exon. The gene construct was purified using CsCl gradient, released from vector, and gel-purified. Itwas microinjected into fertilized mouse oocytes. These oocytes were then transferred to pseudo-pregnant foster mice. The pups born from foster mice were genotyped using PCR, slot blot, and Southern blot techniques. Among 9 mice which showed positive PCR results, only 6 mice transmitted the transgene to thenext generation. Therefore, 6 transgenic lines were established which stably transmitted their transgene totheir progeny. %U https://www.ijbiotech.com/article_6936_480e6518a88c715b47a4425199803d3a.pdf