%0 Journal Article %T Cloning, expression and purification of Clostridium botulinum neurotoxin type E binding domain %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Mousavi, Mir Latif %A Montaser Kouhsari, Shideh %A Nazarian, Shahram %A Rasooli, Iraj %A Amani, Jafar %D 2004 %\ 07/01/2004 %V 2 %N 3 %P 183-188 %! Cloning, expression and purification of Clostridium botulinum neurotoxin type E binding domain %K Botulinum neurotoxin type E %K Binding Domain %K Expression %R %X Botulinum neurotoxins constitute a family of bacterial toxins for botulism syndrome in human. The toxinsbind with high affinity to nerve cells where they cause a complete inhibition and release of neurotransmittersand thereby produce flaccid paralysis. In this study the binding domain of type E neurotoxin was isolated byPCR and expressed in a proper expression vector. The results of this investigation can be used as a toolto study the mechanism of binding of holotoxins. This study is also implicated in antibody production againstbotulism syndrome. %U https://www.ijbiotech.com/article_6909_79f3057bec43722c922ee415a34c6977.pdf