%0 Journal Article %T High-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Golkar, Majid %A Rafati, Sima %A Taslimi, Yasaman %A Taheri, Tahereh %A Doustdari, Fatemeh %A Assmar, Mehdi %D 2004 %\ 07/01/2004 %V 2 %N 3 %P 170-176 %! High-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein %K Toxoplasma gondii %K GRA2 %K Thioredoxin %K E. coli %K Expression %R %X Toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious health problems in immunocompromised individuals and fetus. GRA2 is a dense granule protein of Toxoplasmagondii, which induces strong antibody and T-cell response in human and mice. The purpose of thisstudy was to prepare recombinant GRA2 and evaluate its antigenic properties using infected mice sera. Toreach this point, GRA2 gene was highly expressed as a fusion protein with Thioredoxin (TRX) in Escherichiacoli BL21pLysS strain. The protein was purified in a single step on Ni-NTA affinity column. TRX-GRA2 wasconfirmed by Western blot technique using a monoclonal antibody specific for GRA2. Sera from miceinfected with Toxoplasma gondii showed high reactivity toward GRA2 and the level of IgG2a isotype waspredominant and significantly higher than IgG1.Taken together, TRX-GRA2 might be considered as an idealantigen to be used as a vaccine target as well as diagnostic tool for detection of toxoplasmosis. %U https://www.ijbiotech.com/article_6907_157af281291b57b891b85b3d95a6f140.pdf