%0 Journal Article %T Optimization of Dendrimer Polyamidoamin Electrospun Nanofibers: Preparation and Properties %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Zarei, Zahra %A Mirjalili, Mohammad %A Norooz Kermanshahi, Pouriya %D 2022 %\ 01/01/2022 %V 20 %N 1 %P 36-45 %! Optimization of Dendrimer Polyamidoamin Electrospun Nanofibers: Preparation and Properties %K Dendrimer polyamide amine %K Drug release %K Nanofibers wound dressing %K polyvinyl alcohol %K Tetracycline %R 10.30498/ijb.2021.273730.2996 %X Background: The most common polymers in the treatment of wounds are natural (e.g., polysaccharides, proteins, and peptides) and synthetic polymers (e.g., poly-glycolic acid, polyacrylic acid, polylactic acid, and polyvinyl alcohol) due to their biodegradability, biocompatibility, and their structural resemblance to the macromolecules known to the human body.Objectives: The current study aimed to develop an electrospinning method using the nanofibers of polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC)/polyamide amine (PAMAM)/tetracycline (Tet) to cover the wound. The antibacterial effect of PAMAM was also tested against E. coli and S. aureus bacteria.Materials and Methods: The morphology of the composite nanofiber was studied by a field emission scanning electron microscope. Infrared spectroscopy (FTIR) was used to characterize the nano chemical structure.Results: Nanofibers were evaluated based on the release of different amounts of the antibiotic tetracycline (1%, 3%, 5%, and 7% by weight) while preventing wound infection. The findings indicated that the highest-profile release of all nanofibers occurred early within 12 hours. It was found that nanofiber membranes loaded with 1%, 3%, and 5% tetracycline released drugs for over 28 days, while those containing 7% tetracycline released drugs for more than 14 days.Conclusions: According to the findings related to the drug release of PVA/CMC/15% PAMAM/Tet and surface morphology of the nanofibers, the optimal amount of Tet was 5%. The results of FTIR spectroscopy indicated that the tetracycline and polyamidoamine were successfully placed in nanofibers. %U https://www.ijbiotech.com/article_143252_f381ecf7122cf3d9afbb6bdca80c0c1f.pdf