%0 Journal Article %T Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Mohamadi, Shohreh %A Mehrabi, Maryam %A Sajadimajd, Soraya %D 2021 %\ 08/01/2021 %V 19 %N 3 %P 28-37 %! Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production %K Alkaline %K Bacillus sp %K optimization %K Protease %K Purification %R 10.30498/ijb.2021.247161.2866 %X Background: Proteases play an important role in food, leather, detergent, and medical technologies.Objectives: In the current study, an alkaliphilic solvent-stable thermotolerant metalloprotease was isolated from Bacillus sp. DEM05.Material and Methods: For culture optimization, carbon, and nitrogen sources as well as incubation temperature, pH, and time were examined.Results: Herein the highest outcome for bacterial growth and protease production was obtained after 72 h incubation (pH 7) at 37 °C. DEM05 protease was successfully purified and the specific activity of the protease was 1075 U.mg-1. The purity of the enzyme was verified by SDS-PAGE electrophoresis as a single band of 30 kDa. The optimal activity of the enzyme was at pH 10 and 50 °C. H2O2, SDS, Triton X-100, Zn2+, Co2+, and Cu2+ could increase the protease activity. EDTA inhibited the protease activity, revealed that it can be classified as a metalloprotease. The enzyme was compatible with the water-miscible and water-immiscible organic solvents and proteolyzed several substrates, implying the wide substrate specificity.Conclusions: The results brought convincing evidence that DEM05 protease could be recruited as a novel prevailing protease that can be earmarked on industrial and medical technologies. %U https://www.ijbiotech.com/article_135047_44dea876d1b451ce8d15248531282734.pdf