eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
137
143
7047
Immobilization of Acidithiobacillus Ferrooxidans on Monolithic Packing for Biooxidation of Ferrous Iron
Ehsan Kahrizi
1
Iran Alemzadeh
alemzadeh@sharif.edu
2
Manouchehr Vossoughi
3
Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465, Tehran, I.R. Iran
Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465, Tehran, I.R. Iran
Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465, Tehran, I.R. Iran
The oxidation of ferrous iron (Fe2+) in solution using Acidithiobacillus ferrooxidans has industrial applications exclusively in the regeneration of ferric iron (Fe3+) as an oxidizing agent for the removal of hydrogen sulfide from waste gases, desulfurization of coal, leaching of non-ferrous metallic sulfides and treatment of acid mine drainage. The aim of this investigation was to increase the bio-oxidation rate of ferrous sulfate by using immobilized cells. Rate of Fe2+ oxidation was determined in a packed-bed bioreactor configuration with monolithic particles being used as support material. Biooxidation of ferrous iron by immobilized cells was investigated in repeated batch culture and continuous operation using a laboratory scale packed-bed bioreactor. On this account, effects of process variables such as dilution rate and initial concentrations of Fe2+ on oxidation of ferrous sulfate were consequently investigated. During repeated batch culture, the immobilized-cells were stable and showed high constant iron-oxidizing activities. A maximum Fe2+ oxidation rate of 6.7 g/l/h was achieved at the dilution rate of 2 h-1, while no obvious precipitate was detected in the bioreactor.
https://www.ijbiotech.com/article_7047_a261b66e913a810aae6dae1303dfca21.pdf
Ferrous iron oxidation
Acidithiobacillus ferrooxidans
Packed-bed bioreactor
Dilution rate
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
144
150
7050
Influence of Fungal Enzyme Pre-treatment on Totally Chlorine-free (TCF) Bleaching of Dimethyl Formamid Bagasse Pulp
Amir Sheikhi
1
Ghasem Amoabediny
2
Hamid Rashedi
3
Yaser Ziaie-Shirkolaee
ya_ziaee@yahoo.com
4
Saeed Soltanali
5
Zahra Shams-Haghani
6
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, I.R. Iran
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, I.R. Iran
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, I.R. Iran
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, P.O. Box 11365-4563, Tehran, I.R. Iran
Research Institute of Petroleum Industries, Refining Division, P.O. Box 14665-1998, Tehran, I.R. Iran
Department of Environmental Engineering, Islamiac Azad University, Science and Research Campus, P.O. Box 14665-1998, Tehran, I.R. Iran
A study was carried out on totally chlorine-free (TCF) bleachability of dimethyl formamide (DMF) treated bagasse pulps exposed to CZ-3 and FP 90031-sp strains of white-rot fungus Ceriporiopsis subvermispora. This process involved an oxygen and peroxide stage bleaching sequence. The effect of enzymatic stage on bleachability properties was studied and compared with control pulps, processed without enzyme addition. Final brightness of 79-80% ISO was achieved after complete bleaching. The effects of direct bleaching caused pulp brightening (1.7-1.3% ISO) and delignification (<10%) immediately after the enzymatic stage. Under a peroxide charge of 3% to 9%, the brightness improvement and the bleachability of these pulps were found to be superior to those of the control during all peroxide stages. The selective bleaching of each process was assessed by changes of intrinsic viscosity. Generally higher bleachability and bleaching selectivity of xylanase-treated pulps and the inevitable maximal gain in pulp brightness (or bleach boosting, as a main objective of xylanase application) could be achieved only after the first and second peroxide bleaching stages which then substantially diminished by the end of the sequence.
https://www.ijbiotech.com/article_7050_5acd15ba1184bd17f4c0c6fee9d84b71.pdf
Ceriporiopsis subvermispora
Fungal enzyme
Brightness
Xylanase
Dimethyl formamide pulping
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
151
156
7052
Association of CD24V/V Genotype with Susceptibility and Progression of Multiple Sclerosis in Iranian Population
Mohammad Ronaghi
1
Sadeq Vallian
svallian@medinews.com
2
Masoud Etemadifar
3
Department of Biology, Division of Genetics, Faculty of Science, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran
Department of Biology, Division of Genetics, Faculty of Science, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran
Department of Neurosciences, Isfahan University of Medical Sciences, P.O. Box 81746-73461, Isfahan, I.R. Iran
A single nucleotide polymorphism (SNP) in CD24 has been associated with multiple sclerosis (MS) in a population based study. This SNP results in the replacement of alanine (CD24A) by valine (CD24V) at amino acid 57 in the resulting polypeptide chain. In the current study, the genotyping of this SNP and its contribution to MS in 217 patients and 200 healthy individuals of an Iranian population was investigated. The correlation of the SNP alleles with the progression of the disease was determined using the expanded disability status scale (EDSS) and progression index (PI). The data revealed that individuals with the CD24V/V genotype showed a 2-fold increase in the relative risk of MS compared to patients with the CD24A/V (0.27) and CD24A/A (0.25) genotypes (P = 0.0193, Odds Ratio 2.4882, 95% CI: 1.416-4.3722). Moreover, the progression of the disease in patients with CD24V/V was much faster than other patients that were examined by ANOVA and the least significant difference (LSD) test. However, in the CD24V/V patients LSD analysis was statistically significant (p
https://www.ijbiotech.com/article_7052_2b2f48848169e4a2d3d881217dcc61a0.pdf
Multiple Sclerosis
CD24
Single nucleotide polymorphism
Iranian population
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
157
163
7046
In vitro Induction of Fetal Hemoglobin in Erythroid Cells Derived from CD133 Cells by Transforming Growth Factor-b and Stem Cell Factor
Amir Atashi
1
Masoud Soleimani
soleim_m@modares.ac.ir
2
Saeid Kaviani
kavianis@modares.ac.ir
3
Abbas Hajifathali
4
Ehsan Arefian
5
Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, I.R. Iran
Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, I.R. Iran
Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, I.R. Iran
Department of Hematology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, P.O. Box 16739-111, Tehran, I.R. Iran
Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Company, P.O. Box 14155-3174, Tehran, I.R. Iran
Increased fetal hemoglobin (HbF) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g-globin gene expression. It has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. This study was designed to evaluate the in vitro effects of the stem cell factor (SCF) and transforming growth factor-b (TGF-b) on g-globin gene reactivation of erythroid precursors derived from CD133+ cells in vitro. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis showed increased expression of the g-globin transcript in cell culture groups containing either TGF- b or SCF or both as compared to control (2.2-, 2.7- and 5.5-fold, respectively) (p<0.01). Production of HbF in a differentiated population was demonstrated using flow cytometry. The results of this study suggest that SCF and TGF-b warrant further evaluation as potential therapeutic drugs for the treatment of b-globin gene disorders.
https://www.ijbiotech.com/article_7046_348fa10bf575a08501882d03055e5652.pdf
CD133+ cells
g-globin
Fetal hemoglobin
SCF
TGF-b
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
164
173
7049
Agrobacterium-mediated Transformation of Cotton (Gossypium hirsutum) Using a Synthetic cry1Ab Gene for Enhanced Resistance Against Heliothis armigera
Masoud Tohidfar
gtohidfar@yahoo.com
1
Behzad Ghareyazie
2
Mojgan Mosavi
3
Shohre Yazdani
4
Reyhaneh Golabchian
5
Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, I.R. Iran
Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, I.R. Iran
Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, I.R. Iran
Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, I.R. Iran
Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, I.R. Iran
Cotton (Gossypium hirsutum L.) is an important fiber crop in Iran, cultivated on 150000-200000 ha of land. In Iran the estimated loss due to the insect pest is more than 30%. Traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). In order to produce transgenic cotton resistance to insects, hypocotyl explants were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant binary vector pBI121 containing the cry1Ab gene under the control of CaMV 35S promoter. Neomycin phosphotransferase (nptII) gene was used as a selectable marker. Inoculated tissue sections were placed onto co-cultivation medium. Transformed calli were selected on MS medium containing 50 mg/l of kanamycin and 200 mg/l of cefotaxime. Plantlets were subsequently regenerated from putative transgenic calli. Polymerase chain reaction (PCR) and southern blot analysis were used to confirm the integration of cry1Ab and nptII transgenes into the plant genome. Western immunoblot analysis of proteins extracted from leaves of transgenic plants revealed the presence of an immunoreactive band with a molecular weight (MW) of approximately 67kDa in transgenic cotton lines using the anti-Cry1Ab polyclonal anti-serum. Homozygous T2 plants (Line 61) for the cry1Ab gene showed significantly higher levels of insect resistance against Heliothis armigera larvae compared with the control plants. Transgenic plants are currently grown in the greenhouse and will be crossed with Iranian cotton breeding lines.
https://www.ijbiotech.com/article_7049_301d29699a5c79a79ae5ffa9d440e920.pdf
Agrobacterium tumefaciens
Bacillus thuringiensis
cotton
Heliothis armigera
Insect resistance
Genetic transformation
cry1Ab
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
174
180
7043
Intracellular Localization of FLAG-Peroxisomal Protein in Chinese Hamster Ovary (CHO) Cells
Malihe Nazari Jahantigh
1
Kamran Ghaedi
2
Mohamad Hossein Nasr Isfahani
3
Somayeh Tanhaei
4
Farzaneh Rabiee
5
Khadijeh Karbalaei
6
Maryam Ostad Sharif
7
Marzieh Nematollahi
8
Hossein Baharvand
9
Shahnaz Razavi
10
Mehran Miroliaei
11
Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran
Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran
Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran
Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran
Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran
Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran
Department of Basic Medical Sciences, School of Dentistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran
Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran
Cell Sciences Research Center, Royan Institute, P.O. Box 19395-4644, Tehran, I.R. Iran
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, P.O. Box 81746-73471, Isfahan, I.R. Iran
Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was constructed using the method of splicing by overlap extension polymerase chain reaction (SOE PCR) and inserted into the pUcD2SRaMCSHyg eukaryotic expression vector. To investigate the intracellular localization of the PEP protein that was linked to the FLAG tandem, the constructed plasmid was used for transient transfection of the Chinese hamster Ovary (CHO) cells. The CHO cells that were transfected with the recombinant plasmid showed peroxisomal localization of FLAG-PEP as was previously shown for catalase.
https://www.ijbiotech.com/article_7043_f5ee941cdccd5b165f78f6607f99df8a.pdf
PEP cDNA
Peroxisomes
PTS1 signal
Transfection
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2008-07-01
6
3
181
185
7042
Detection of the ectC Gene in Halomonas Strains by Polymerase Chain Reaction
Maryam Zanjirband
mzanjirband@yahoo.com
1
Nasser Golbang
2
Ruha Kasra Kermanshahi
3
Department of Biology, Faculty of Science, Isfahan University, P.O. Box 117, Isfahan, I.R. Iran
Department of Biology, Faculty of Science, Isfahan University, P.O. Box 117, Isfahan, I.R. Iran
Department of Biology, Faculty of Science, Isfahan University, P.O. Box 117, Isfahan, I.R. Iran
1, 4, 5, 6-Tetrahydro-2-methyl-4-pyrimidine carboxylic acid (ectoine) is an excellent osmoprotectant. Ectoine and hydroxyl ectoines are of great significance to the biotechnology industry, thus the detection and isolation of ectoine producing bacteria is of great importance. Hence, this study involved the detection of the ectC gene (encoding ectoin synthase enzyme) using polymerase chain reaction (PCR) method. For isolation of moderately halophilic bacteria, environmental samples were collected from various sites of a tannery factory in Isfahan, and the Persian Gulf. A synthetic broth medium was used and the optimum concentration of salt (NaCl) was determined by the microtitre plate method. Based on the alignment of relevant ectC gene sequences available in the GenBank which included sequences from 24 validly described Halomonas species, putative genus-specific primers were designed. Primers were designed in such a way to amplify a 277bp region of the ectC gene in the putative Halomonas strains. PCR analysis showed that 75% (34/45) of the samples belong to the Halomonas genus capable of producing ectoine synthase. Ectoine primer pair was designed to amplify all Halomonas species capable of producing ectoine synthase.
https://www.ijbiotech.com/article_7042_33963dd06641af43635b6e8852108bb1.pdf
Halotolerant/halophilic
Ectoine
PCR
salt
Halomonas