eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
1
16
6980
Class-Pi of glutathione S-transferases
Somaieh Kazemnejad
1
Yusef Rasmi
2
Roya Sharifi
3
Abdolamir Allameh
allameha@ modares.ac.ir
4
Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran.
Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran.
Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran.
Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran.
Class-Pi of glutathione s-transferases (GST-Pi) is the specific form of GSTs that are known to participate particularly in the mechanisms of resistance to drugs and carcinogens. This class of the enzyme is referred to as class-P or class-Pi or class π. The accepted terminology in this review article is class-Pi. In this article following a brief description of identified molecular forms of GSTs, we focus on GST-Pi. We review new findings about the structure and regulation of GST-Pi gene. Then, the role of GST-Pi in liver damage, oxidative stress, carcinogenesis and drug resistance are discussed. Also, the presence of common genetic polymorphism,hypermethylation in GST-Pi gene and the consequences GST-Pi knock out is regarded.
https://www.ijbiotech.com/article_6980_1253907f5603fe4fae8ccd0bccbe78f5.pdf
Glutathione S-transferase Pi
Drug resistance
carcinogenesis
oxidative stress
Polymorphism
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
17
25
7006
In silico and in vitro investigations on cry4a and cry11a toxins of Bacillus thuringiensis var israelensis
Kaiser Jamil
1
Suvarchala Devi
2
Muhummad Khan
3
Department of Genetics, Bhagwan Mahavir Hospital and Research Center, Hyderabad, A.P, India.
Department of Genetics, Bhagwan Mahavir Hospital and Research Center, Hyderabad, A.P, India.
Department of Genetics, Bhagwan Mahavir Hospital and Research Center, Hyderabad, A.P, India.
In the present study we attempted to correlate the structure and function of the cry11a (72 kDa) and cry4a (135 kDa) proteins of Bacillus thuringiensis var israelensis. Homology modeling and secondary structure predictions were done to locate most probable regions for finding helices or strands in these proteins. The JPRED (JPRED consensus secondary structure prediction server) secondary structure predictions were chosen for its ability to predict with high accuracies. The homology model predicted by CPH (CPH Homology Modelling server) modeler showed a distinct region of helices and sheets. The membrane spanning helices werepredicted using TOPPRED (TOPPRED Topology prediction of membrane proteins server); these helices are known to play crucial role in cell lyses. The role of one such segment corresponding to amino acids 132-150 of cry4a protein, which had a large hydrophobic moment, was elucidated. The Circular Dichroism spectra of the peptide showed helical structure in methanol and β sheet structure in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). The biological activity of this peptide was investigated. The peptideshowed weak hemolytic activity in vitro. This may be due to the synthetic peptide used rather than the whole molecule in the native environment. The ability of the peptide and the alkali solubilized crystal proteins to perturb the synthetic membrane was investigated using carboxyflourescein trapped liposomes. The leakage caused by alkali solubilized extract was double than the leakage caused by synthetic peptide. In case of alkali solubilized extract, various osmoprotectants were seen to delay lytic activity. Thus it is clear that the cry proteins are highly active and lethal in their native state. Not only the membrane spanning segments butthe whole molecule plays a crucial role in lysis.
https://www.ijbiotech.com/article_7006_243e6c5108ae897953e02aa6d9b897da.pdf
Bacillus thuringiensis
Cry proteins
Homology modeling
Larvicidal activity
Amphipathic helices
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
26
35
6977
Purification and characterization of two acid phosphatases from germinating peanut (Arachis hypogaea) seed
Jean Tia Gonnety
1
Sebastien Niamke
2
Betty Meuwiah Faulet
3
Eugène Jean-Parfait N’guessan Kouadio
4
Lucien Patrice Kouame
5
Laboratoire de Biochimie et Technologie des Aliments de l’Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l’Universite d’Abobo-Adjamé, 02, BP 801 Abidjan 02, Côte d’Ivoire.
Laboratoire de Biotechnologies, Filière Biochimie-Microbiologie de l’Unité de Formation et de Recherche en Biosciences de l’Université de Cocody-Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire.
Laboratoire de Biochimie et Technologie des Aliments de l’Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l’Universite d’Abobo-Adjamé, 02, BP 801 Abidjan 02, Côte d’Ivoire.
Laboratoire de Biochimie et Technologie des Aliments de l’Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l’Universite d’Abobo-Adjamé, 02, BP 801 Abidjan 02, Côte d’Ivoire.
Laboratoire de Biochimie et Technologie des Aliments de l’Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l’Universite d’Abobo-Adjamé, 02, BP 801 Abidjan 02, Côte d’Ivoire.
The maximum acid phosphatasic activity was detected in peanut seed at the 5th day of germination. At least, two acid phosphatases were purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-100 HR, and Phenyl-Sepharose HP to apparent homogeneity from five days old cotyledon of peanut after germination. These isoenzymes, designated peanut cotyledon acid phosphatase 1 and 2 (PCAP 1 and PCAP 2), had native molecular weights of approximately 27.5 and 24 kDa by gel permeation, respectively. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)of PCAP 1 and PCAP 2 resolved a single protein band (each) that migrated to approximately 27 and 29 kDa,respectively. Thus, these acid phosphatases likely function as a monomer. The two isoenzymes had a similar optimum temperature (55°C), two closely optima pH (5.6 and 5.0), and appeared to be stable in the presence of some detergents such as Triton X-100, Nonidet P-40, Taurocholic acid sodium salt, Polyoxyethylene-9-lauryl ether as well as Mg2+, Sr2+,Fe3+ and Ba2+. Substrate specificity indicated that PCAP 1 and PCAP 2 hydrolyzed a broad range of phosphorylated substrates. However, natural substrates such as ADP, ATPand phenylphosphate had the highest rate of hydrolysis for the two isoenzymes.
https://www.ijbiotech.com/article_6977_cdd963f4077e68f8a04e7a45b8a19b14.pdf
Acid Phosphatase
Arachis hypogaea
Cotyledon
Germination
Peanut
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
36
44
6974
Genetic diversity of Iranian and some of European grapes revealed by microsatellite markers
Javad Najafi
1
Leila Alipanah
2
Behzad Ghareyazie
ghareyazie@yahoo.com
3
Seyed Abulgasem Mohammadi
4
Ali Hagh Nazari
5
Patric This
6
Agricultural Biotechnology Research Institute of Iran (ABRII). Seed and Plant Improvement Institute Campus, Mahdasht Road, Karaj, Iran. P.O. Box 31535-1897
Agricultural Biotechnology Research Institute of Iran (ABRII). Seed and Plant Improvement Institute Campus, Mahdasht Road, Karaj, Iran. P.O. Box 31535-1897
Agricultural Biotechnology Research Institute of Iran (ABRII). Seed and Plant Improvement Institute Campus, Mahdasht Road, Karaj, Iran. P.O. Box 31535-1897
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, I.R. Iran.
Agricultural Biotechnology Research Institute of Zanjan University, Zanjan, I. R. Iran.
UMR DGPC, INRA-ENSAM, 2 Place viala, 34060 Montpollier, France.
In order to characterize Iranian grape (Vitis vinifera L.) germplasm, 136 genotypes were collected from five grape growing regions (Azarbaijan, Qazvin, Kordestan, Khorasan and Fars) and genotyped along with 36 European cultivars using 9 sequence tagged microsatellite sites (STMS) markers. The used set of markers could distinguish all 172 genotypes under study. Altogether 84 polymorphic alleles were observed detected all the genotypes, with an average of 9.33 and 5.81 effective alleles per locus. The expected heterozygosityvalues were higher than those observed for all the loci. This could probably be due to the occurrence of null alleles at these loci. The usefulness of this set of markers for genotype distinction was assessed as probability of identity (PI). The estimated total PI value over all the geographic regions for this set of markers was estimated to be 5.67×10-9. Comparison of samples from different grape growing regions of Iran and Europe based on various parameters using allelic data revealed similar level of genetic variation. Analysis ofmolecular variance (AMOVA) indicated significant difference between samples, however, no difference was observed between the Iranian and European groups. Genetic differentiation among samples based on Fst in most pairwise comparisons was significant. Cluster analysis based on coancestry coefficient matrix and principal coordinate analysis confirmed the result of AMOVA and Fst analysis.
https://www.ijbiotech.com/article_6974_39b09875edc2848b9d5aa081a3fcb9d9.pdf
grape
Sequence Tagged Microsatellite Sites (STMS)
Genetic variation
Analysis of Molecular Variance (AMOVA)
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
45
53
6971
Liquid fuel production from synthesis gas via fermentation process in a continuous tank bioreactor (CSTBR) using Clostridium ljungdahlii
Habibollah Younesi
1
Ghasem Najafpour
najafpour@nit.ac.ir
2
Abdul Rahman Mohamed
3
Department of Environment, Faculty of Natural Resources and Marine Sceinces, Tarbiat Modares University, Noor, I.R. Iran.
Faculty of Chemical Engineering, Noshirvani Institute of Technology, University of Mazandaran, Babol, I.R.Iran.
School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, Nibong Tebal, 14300 Penang, Malaysia.
The potential bioconversion of synthesis gas (syngas) to fuels and chemicals by microbial cell has attracted considerable attention in past decade. The feasibility of enhancing syngas bioconversion to ethanol and acetate using Clostridium ljungdahlii in a continuous tank bioreactor (CSTBR), kinetics and mass transfer coefficient of carbon monoxide (CO) utilization were evaluated. Two different types of syngas and pure CO were used for CO limitation, in order to achieve high bioconversion of syngas in a single bioreactor. The CO conversion increased with an increase in gas flow rate and agitation speed. For a gas flow rate of 14 ml/min and an agitation rate of 550 rpm, the cell concentration and conversion of pure CO were 1.92 g/l and 80%,respectively. The cell concentration also increased with an increase in CO percentage in the gas phase. Maximum cell dry weight of 2 g/l and CO conversion of 93% were achieved with 70% CO blended syngas at a gas flow rate of 14 ml/min and agitation rate of 500 rpm. The total amounts of ethanol and acetate concentrations were approximately 11 g/l. The mass transfer coefficient was calculated for the culture of C.ljungdahlii with pure CO in the CSTBR. The maximum mass transfer coefficient (KLa) was 135 h-1 at an agitation speed of 550 rpm. The KLa correlation based on various gas flow rates and agitation speeds was fitted with experimental data and the predicted model was in good conformity with the obtained data.
https://www.ijbiotech.com/article_6971_d55ae1a03c281f84f5deee9f2a33b85d.pdf
Biofuels
Clostridium ljungdahlii
Mass Transfer Coefficient
Synthesis gas
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
45
53
6892
Effects of Genotype and Cotyledon Section on Organogenesis in Sunflower
https://www.ijbiotech.com/article_6892_db59260cce0b871c7b2bb780eee305db.pdf
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
54
60
6985
Fabrication of porous hydroxyapatite-gelatin scaffolds crosslinked by glutaraldehyde for bone tissue engineering
Mehdi Kazemzadeh Narbat
1
Mehran Solati Hashtjin
2
Mohammad Pazouki
m.pazouki@merc.ac.ir
3
Department of Biomaterials, Faculty of Biomedical Engineering, Islamic Azad University, Science and Research Unit, Tehran, I.R. Iran.
Department of Bioceramics, Faculty of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
Department of Energy, Materials and Energy Research Center (MERC), P.O. Box 31787-316, Karaj, I.R. Iran.
In this study, to mimic the mineral and organic components of natural bone, hydroxyapatite[HA] and gelatin[GEL] composite scaffolds were prepared using the solvent-casting method combined with a freeze drying process. Glutaraldehyde[GA] was used as a cross linking agent and sodium bisulfite was used as an excess GA discharger. Using this technique, it is possible to produce scaffolds with mechanical and structural properties close to those of the natural trabecular bone. The prepared scaffold has an open, interconnected porous structure. It was found that the GEL/HA ratio with a 50 wt% (weight percent) HA has the compressive modulus, the ultimate compressive stress and elongation similar to those for the trabecular bone. The chemical bonding and the microstructure of the composites were investigated by FT-IR (Fourier Transform Infra Red), SEM (Scanning Electron Microscopy) and Light microscopy, indicating the presence of bonds between Ca2+ ions of HA and R-COO- ions of GEL in the HA-GEL composite scaffolds. It was found that the addition of HA content can reduce the water absorption and porosity of scaffold. The porosity and the apparent density of 50 wt% HA scaffold were also calculated. The biological responses of scaffolds were examined in L929 fibroblast cell culture, showed partially proliferation of cells around and on the composite surface.
https://www.ijbiotech.com/article_6985_68b291805d4fbbcd7c3dd0570c6b7042.pdf
Scaffolds
Hydroxyapatite
Gelatin
Composite
Bone tissue engineering
Glutaraldehyde
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
61
63
6979
Differentiation of virulent and non-virulent Newcastle disease virus isolates using RT-PCR
Sara Baratchi
1
Seyed Ali Ghorashi
2
Masoud Hosseini
3
Seyed Ali Pourbakhsh
4
Department of Microbiology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, I.R. Iran.
Department of Microbiology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, I.R. Iran.
Department of Biology, Faculty of Science, Shahid Beheshti University, Evin, Tehran, I.R. Iran.
Department of Poultry Disease, Razi Vaccine and Serum Research Institute, P.O. Box 31975-148 Karaj, I.R. Iran.
Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates were tested by this technique.RT-PCR was performed using two sets of primers chosen from a section of the F gene. The PCR product wascloned in to a pTZ57R/T vector and sequenced. The sequence data confirmed the specificity of the test. Detection of viral virulence was determined based on the amplification of PCR products. The above optimized RT-PCR produce can be used to confirm the diagnosis of Newcastle disease within 24 hrs using RNA isolated directly from tissue homogenate or passaged in SPF (Specific Pathogen Free) embryonated eggs.
https://www.ijbiotech.com/article_6979_95eb4ebd74023dfa4c1b02673932a11a.pdf
Newcastle disease virus
Virulent strains
Nonvirulent strains
Differential detection
RT-PCR
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2003-10-01
4
1
64
68
6973
Role of mitochondria in Ataxia-Telangiectasia: Investigation of mitochondrial deletions and Haplogroups
Massoud Houshmand
housbmand.m@gmail.com
1
Mohammad Hossein Sanati
z.elyasigorji@gmail.com
2
Baharak Hooshiar Kashani
3
Mehdi Shafa Shariat Panahi
4
Mohammad Mehdi Banoei
5
Anna Isaian
6
Mostafa Moin
7
Abolhasan Farhoudi
8
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155- 6343, Tehran, I.R. Iran.
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155- 6343, Tehran, I.R. Iran.
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155- 6343, Tehran, I.R. Iran.
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155- 6343, Tehran, I.R. Iran.
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155- 6343, Tehran, I.R. Iran.
Department of Allergy and Clinical Immunology, Children Medical Center, Tehran University of Medical Sciences, P.O. Box 14185-863, Tehran, I.R. Iran
Department of Allergy and Clinical Immunology, Children Medical Center, Tehran University of Medical Sciences, P.O. Box 14185-863, Tehran, I.R. Iran.
Department of Allergy and Clinical Immunology, Children Medical Center, Tehran University of Medical Sciences, P.O. Box 14185-863, Tehran, I.R. Iran.
Ataxia-Telangiectasia (AT) is a rare human neurodegenerative autosomal recessive multisystem disease that is characterized by a wide range of features including, progressive cerebellar ataxia with onset during infancy, occulocutaneous telangiectasia, susceptibility to neoplasia, occulomotor disturbances, chromosomal instability and growth and developmental abnormalities. Mitochondrial DNA (mtDNA) has the only non-coding regions at the displacement loop (D-loop) region that contains two hypervariable segments (HVS-I and HVS-II) with high polymorphism. We investigated mt-DNA deletions and haplogroups in AT patients. In this study, 24 Iranian patients suffering from AT and 100 normal controls were examined. mt-DNA was extracted from whole bloodand examined by 6 primers for existence of mitochondrial deletions. We also amplified and sequenced the mtDNA HVS-I by standard sequencing techniques. mtDNA deletions were observed in 54.1% (13/24) of patients (8.9 kb deletion in all samples, 5.0 kb in one and 7.5 kb in two patients), representing mtDNA damage which may be due to oxidative stress in mitochondria. Our results showed that there is no association between mtDNA haplogroups and AT. This data may indicate involvement of mitochondrial damage in the pathogenesis of AT.
https://www.ijbiotech.com/article_6973_891984335667b81d3dd8383bb3a1cd90.pdf
Ataxia-Telangiectasia
Mitochondrial DNA Deletion
Haplogroup