@article { author = {Mousavi Gargari, Seyed Latif and Rasooli, Iraj and Valipour, Ebrahim and Basiri, Mohsen and Nazarian, Shahram and Amani, Jafar and Farhadi, Nima}, title = {Immunogenic and protective potentials of recombinant receptor binding domain and a C-terminal fragment of Clostridium botulinum neurotoxin type E}, journal = {Iranian Journal of Biotechnology}, volume = {9}, number = {3}, pages = {181-187}, year = {2011}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding domain) and the c-terminal quarter of binding domain (HcQ), were cloned in pET-28a vector and over-expressed in E. coli BL21 (DE3) cells.These recombinant proteins were purified by affinity Ni-NTA column (under native condition). Mice werevaccinated with 2 μg of purified proteins, respectively; at step one with complete adjuvant, steps two andthree with incomplete adjuvant and step four only with phosphate buffered saline (PBS). Enzyme-linkedimmunosorbent assay (ELISA) has been performed with mice serum samples 14 days following their thirdand final vaccination. Binding activity of the purified proteins to ganglioside and synaptotagmin II was analyzed by ELISA. The results showed that HcQ and Hc could bind with ganglioside. Based on challengeexperiments it was revealed that HcQ, Hc and BoNT/E toxoid could give protections in mice challenged with102 , 104 and 105 minimum lethal dose (MLD) dose of BoNT/E.}, keywords = {Botulinum Neurotoxin,Recombinant Vaccine,Binding Domain,Synthetic Gene}, url = {https://www.ijbiotech.com/article_7152.html}, eprint = {https://www.ijbiotech.com/article_7152_fcba998c4fd629857b727b8fa5611d56.pdf} }