@article { author = {Sarikhani, Mohammad Reza and Malboobi, Mohammad Ali and Aliasgharzad, Nasser and Greine, Ralf and Yakhchali, Bagher}, title = {Functional screening of phosphatase-encoding genes from bacterial sources}, journal = {Iranian Journal of Biotechnology}, volume = {8}, number = {4}, pages = {275-279}, year = {2010}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Phosphatase (APase) enzymes including phytases have broad applications in diagnostic kits, poultryfeeds, biofertilizers and plant nutrition. Because of high levels of sequence diversity among phosphatases,an efficient functional screening method is a crucial requirement for the isolation of the encodinggenes. This study reports a functional cloning screening method for the isolation of APase-encoding genesfrom bacterial genomic libraries in a medium containing a chromogenic substrate. The method was optimizedto distinguish the desired signal from the background chromosomal APase activity. This screening method led to the isolation of two novel APase-encoding genes from Pseudomonas putida with no similarities to the known genes in the databases, indicating successful implementation of the developed method.}, keywords = {Pseudomonas putida,Phosphatase,Phytase,Phytate,BCIP}, url = {https://www.ijbiotech.com/article_7112.html}, eprint = {https://www.ijbiotech.com/article_7112_9f7825604d12b9afcad2bc63307c4101.pdf} }