@article { author = {Gharanjik, Shahrokh and Moieni, Ahmad and Mousavi, Amir and Alizadeh, Houshang}, title = {Optimization of Transient Expression of uidA Gene in Androgenic Embryos of Wheat (Triticum aestivum L. cv. Falat) via Particle bombardment}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {207-213}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These  embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0.6 mm, 1 mm and 1.6 mm), DNA and microcarrier concentrations (0.5 mg of DNA with 150 μg of gold particles or 1.0 μg of DNA with 300 mg of gold particles/bombardment) and bombardment numbers (1x (single) and 2x (double)) were assessed. The effect of high osmoticum in the bombardment medium (0.3 M mannitol and 0.4 M maltose) and the age of embryos were also evaluated.  Optimal expression in MDEs was obtained using the following conditions of double bombardment at 1350 psi, 9 cm target distance, a 1 mm gold particle size, 1.0 mg of DNA with 300 mg of gold particles/bombardment, and osmotic pretreatment of 4-6 weeks old embryos using 0.4 M maltose for 6 h before and 16 h after bombardment. The optimized transformation protocol presented in this study is expected to improve devalopment of commercial transgenic wheat lines expressing desirable agronomic traits.}, keywords = {Hexaploid wheat,Transient gene expression,GUS,Particle bombardment,Androgenic embryos}, url = {https://www.ijbiotech.com/article_7038.html}, eprint = {https://www.ijbiotech.com/article_7038_5fc8dcea5e821c748d5ca561a33223ba.pdf} }