@article { author = {Hushiarian, Roozbeh and Roayaei, Mohammad and Galehdari, Hamid and Seyfiabad Shapouri, Masoud Reza}, title = {Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells}, journal = {Iranian Journal of Biotechnology}, volume = {5}, number = {4}, pages = {246-250}, year = {2007}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a eukaryotic expression plasmid, pCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 cell line and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV.}, keywords = {Infectious bursal disease,IBDV,VP2,eukaryotic expression,pCDNA4,COS-7 cell line}, url = {https://www.ijbiotech.com/article_7016.html}, eprint = {https://www.ijbiotech.com/article_7016_39c7f9a3cd17e71cb4903dd362fd8331.pdf} }