@article { author = {Shahinsaz, Leila and Sabahi, Farzaneh and Karimi, Mohsen and Behzadian, Farida and Alavian, Seyed Moayed and Zand, Vahid}, title = {Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR}, journal = {Iranian Journal of Biotechnology}, volume = {4}, number = {3}, pages = {174-179}, year = {2006}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen (HDAg) C-termini were used for genotyping. The results showed that 63.6% (23 of 36) of (HDAb) positive serum samples (as determined by ELISA) were also positive for HDV-RNA. Sequencing and phylogenic analysis of three Iranian HDV isolates revealed the most homology (93%) with an Italian isolate indicating a close relationship and probably a common origin for these isolates.}, keywords = {Hepatitis Delta virus,RT-PCR,phylogenetic tree,sequencing,Iran}, url = {https://www.ijbiotech.com/article_6976.html}, eprint = {https://www.ijbiotech.com/article_6976_eb77294d2658b69d0d7cb4bd55115ca7.pdf} }