@article { author = {Haddad, Raheem and Morris, Karl and Buchanan-Wollaston, Vicky}, title = {Expression Analysis of Genes Related to Oxidative Protection During Senescence in Brassica napus}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {269-278}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Leaf senescence is the sequence of events leading to cellular disassembly and mobilization of releasedmaterials that cause accumulation of reactive oxygen species. DNA sequencing of one of senescence-isolatedcDNA, LSC650, showed a high level of similarity with a catalase gene of Arabidopsis. Transcript level ofthis isoform was highly increased during senescence. The enzyme activity of catalase was also assayed duringdifferent developmental stages in Brassica napus and showed a high level of activity during last part ofleaf senescence. Both kinetic assay and northern analysis exhibit that catalase has the potential to playa significant role in the cell defense against produced hydrogen peroxide as part of the macromoleculesbreakdown. Gene expression pattern was characterized by mRNA hybridization with several antioxidantcDNA clones. Transcript levels of discussed genes were markedly increased during senescence stages.We conclude that the major part of the mRNA changes observed during senescence stages of leaves is connected with leaf senescence, whereas free radicalrelated transcripts appear to protect cells from oxidativedamage.}, keywords = {Active oxygen species,free radicals,Transcription,Northern analysis,Leaf senescence,Brassica napus}, url = {https://www.ijbiotech.com/article_6919.html}, eprint = {https://www.ijbiotech.com/article_6919_150c3b834863056808c99363641f68c3.pdf} }