@article { author = {Yaghoobi, Maliheh and Hashemi-Najafabadi, Sameereh and Soleimani, Masoud and Vasheghani-Farahani, Ebrahim and Mousavi, Seyyed Mohammad}, title = {Osteogenic Differentiation and Mineralization on Compact Multilayer nHA-PCL Electrospun Scaffolds in a Perfusion Bioreactor}, journal = {Iranian Journal of Biotechnology}, volume = {14}, number = {2}, pages = {41-49}, year = {2016}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {10.15171/ijb.1382}, abstract = {Background: Monolayer electrospun scaffolds have already been used in bone tissue engineering due to their high surface-to-volume ratio, interconnectivity, similarity to natural bone extracellular matrix (ECM), and simple production. Objectives: The aim of this study was to evaluate the dynamic culture effect on osteogenic differentiation and mineralizationi into a compact cellular multilayer nHA-PCL electrospun construct. The dynamic culture was compared with static culture. Materials and Methods: The calcium content, alkaline phosphatase (ALP) activity and cell viability were investigated on days 3 and 7. Results: When the dynamic culture compared to static culture, the mineralization and ALP activity were increased in dynamic culture. After 7 days, calcium contents were 41.24 and 20.44 mg.(cm3)-1, and also normalized ALP activity were 0.32 and 0.19 U.mg-1 in dynamic and static culture, respectively. Despite decreasing the cell viability until day 7, the scanning electron microscopy (SEM) results showed that, due to higher mineralization, a larger area of the construct was covered with calcium deposition in dynamic culture. Conclusions: The dynamic flow could improve ALP activity and mineralization into the compact cellular multilayer construct cultured in the perfusion bioreactor after 7 days. Fluid flow of media helped to facilitate the nutrients transportation into the construct and created uniform cellular construct with high mineralization. This construct can be applied for bone tissue engineering.}, keywords = {Mineralization,Electrospun scaffolds,Multilayer construct,Osteogenic differentiation,Perfusion bioreactor}, url = {https://www.ijbiotech.com/article_14138.html}, eprint = {https://www.ijbiotech.com/article_14138_8bce3668921ec632085be849dbd5ffe5.pdf} }