@article { author = {Talesh Sasani, Soheila and M. Soltani, Bahram and Mehrabi, Rahim and Samavatian, Hossein and Padasht-Dehkaei, Fereidoun}, title = {Expression Alteration of Candidate Rice MiRNAs in Response to Sheath Blight Disease}, journal = {Iranian Journal of Biotechnology}, volume = {18}, number = {4}, pages = {39-46}, year = {2020}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {10.30498/IJB.2020.2451}, abstract = {Background: MicroRNAs, as small non-coding RNAs, are recently reported to be involved in plant defense system against pathogens including fungi.Objective: In this research, it was intended to investigate candidate susceptible rice (Oryza Sativa) Osa-miRNA expression alteration following the infection by Rhizoctonia solani.Materials and Methods: To this aim, literature review suggested eight conserved plant miRNAs that are involved in other plant-pathogen interactions. Then, sixty days old rice plants (Hashemi, susceptible cultivar) were inoculated with R. solani and candidate miRNA expression alterations were investigated 2 hpi (hours post inoculation), 2 dpi (days post inoculation) and 6 dpi.Results: RT-qPCR analysis suggested four subgroups of candidate miRNAs based on the time of their responses to the pathogenesis of R. solani. While Osa-miR-156 was early-responsive, Osa-miR159 was the last-responsive and Osa-miR167, Osa-miR171, Osa-miR408, and Osa-miR444 were late responsive to R. solani infection. Osa-miR166 and Osa-miR393 were non-responsive to this infection, compared to the mock-inoculated control group. Consistently, Os-SPL3 and Os-MADS known target genes were expressed in reverse correlation to Osa-miR156 and Osa-miR444, respectively.Conclusions: From these data, it is suggested that both early (Osa-miR-156) and late (Osa-miR167, Osa-miR171, OsamiR408, Osa-miR444) responsive miRNAs might be involved in R. solani infection in rice plants.}, keywords = {miRNA,Rhizoctonia solani,Rice plant}, url = {https://www.ijbiotech.com/article_111374.html}, eprint = {https://www.ijbiotech.com/article_111374_cfccb93c9389b5a1aa4ba955ff03bd5a.pdf} }