@article { author = {Azizi, Abdulbaset and Mozafari, Javad and Shams-bakhsh, Masoud}, title = {Phenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {199-206}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance to an Iranian isolate of TYLCV. Plants were inoculated using whiteflies (Bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral DNA amplification. All accessions of S. lycopersicum had demonstrated various degrees of disease symptoms. However, all six accessions of S. peruvianum were resistant and remained symptomless. Phenotypic evaluation was confirmed by amplification of a 670bp TYLCV DNA fragment in all tested accessions of S. lycopersicum. Based on both phenotypic and molecular evaluations, no accession provided complete resistance to TYLCV, whereas nine accessions were assessed as tolerant. The high level of resistance noted in whitefly inoculated accessions of S. peruvianum was not observed in graft inoculated plants of these accessions. The TYLCV DNA fragment was detected five weeks post-inoculation when plants were inoculated by grafting. These results suggested that accessions of S. peruvianum may be merely resistant to vector inoculation of TYLCV.}, keywords = {Molecular screening,Tomato,TYLCV,Virus resistance}, url = {https://www.ijbiotech.com/article_7037.html}, eprint = {https://www.ijbiotech.com/article_7037_dd9cda0e0df121a5d3204158dab23f4e.pdf} } @article { author = {Gharanjik, Shahrokh and Moieni, Ahmad and Mousavi, Amir and Alizadeh, Houshang}, title = {Optimization of Transient Expression of uidA Gene in Androgenic Embryos of Wheat (Triticum aestivum L. cv. Falat) via Particle bombardment}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {207-213}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These  embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0.6 mm, 1 mm and 1.6 mm), DNA and microcarrier concentrations (0.5 mg of DNA with 150 μg of gold particles or 1.0 μg of DNA with 300 mg of gold particles/bombardment) and bombardment numbers (1x (single) and 2x (double)) were assessed. The effect of high osmoticum in the bombardment medium (0.3 M mannitol and 0.4 M maltose) and the age of embryos were also evaluated.  Optimal expression in MDEs was obtained using the following conditions of double bombardment at 1350 psi, 9 cm target distance, a 1 mm gold particle size, 1.0 mg of DNA with 300 mg of gold particles/bombardment, and osmotic pretreatment of 4-6 weeks old embryos using 0.4 M maltose for 6 h before and 16 h after bombardment. The optimized transformation protocol presented in this study is expected to improve devalopment of commercial transgenic wheat lines expressing desirable agronomic traits.}, keywords = {Hexaploid wheat,Transient gene expression,GUS,Particle bombardment,Androgenic embryos}, url = {https://www.ijbiotech.com/article_7038.html}, eprint = {https://www.ijbiotech.com/article_7038_5fc8dcea5e821c748d5ca561a33223ba.pdf} } @article { author = {Seifzadeh, Sara and Hassan Sajedi, Reza and Sariri, Reyhaneh}, title = {Isolation and Characterization of Thermophilic Alkaline Proteases Resistant to Sodium Dodecyl Sulfate and Ethylene Diamine Tetraacetic Acid from Bacillus sp. GUS1}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {214-221}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Thermophilic Bacillus sp. GUS1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. The enzymes were stable in the alkaline pH range (8.0-12.0), with the optimum temperature and pH range of the proteases being 70ºC and 6.0-12.0, respectively. All three proteases were also highly stable at 70ºC. After 60 min of incubation at 70ºC, the enzymes retained 100% of their original activities. Enzymes were mostly inhibited by phenylmethylsulfonyl fluoride (PMSF), however 80-90% enzyme activities were retained in presence of 2-mercaptoethanol and iodoacetate. Addition of SDS and ethylene diamine tetraacetic acid (EDTA) also marginally influenced protease activities, but addition of Ca2+ to the proteases did not bring about any change. The results suggeste that most of these proteases were not metalloproteases, but Ca2+-independent serine alkaline proteases.}, keywords = {Bacillus sp,Alkaline serine proteases,Thermostability Resistance to EDTA and SDS}, url = {https://www.ijbiotech.com/article_7039.html}, eprint = {https://www.ijbiotech.com/article_7039_6eb2271b1fe56ae87dd0756591916e81.pdf} } @article { author = {Saeedinia, Alireza and Shamsara, Mehdi and Zeinoddini, Mehdi and Sadeghi, Vahid and Maghsoudi, Nader}, title = {Evaluation of Nucleic Acid Sequence Based Amplification (NASBA) and Reverse Transcription Polymerase Chain Reaction for Detection of Coxsackievirus B3 in Cell Culture and Animal Tissue Samples}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {222-228}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Enteroviruses are the causative agents of a number of diseases in humans. Group B coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. Genomic data of enteroviruses has allowed developing new molecular approaches such as Nucleic Acid Sequence Based Amplification (NASBA) for detection of such viruses. In this study, coxsackievirus B3 (CVB3) was detected in virus-infected cell culture and specimens of artificially infected mice with specific primers using Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and NASBA techniques. According to the results, both techniques could be used for the detection of viruses in cell culture and artificially infected animals. NASBA reaction was simpler to perform than RT-PCR. The only variable factor that had to be optimized with NASBA is KCl concentration. The optimal concentration of KCl was determined as 90 mM. Serial dilutions of 1 mg of total RNA showed that both RT-PCR and NASBA could detect the virus at 10-5 dilution. Analyses of heart and spleen samples from infected animals were positive for presence of Coxsackievirus B3 with both RT-PCR and NASBA. In conclusion, NASBA offers some advantages over RT-PCR and is a suitable alternative technique for the sensitive detection of CVB3 in contaminated samples.}, keywords = {Coxsackievirus B3,NASBA,RT-PCR}, url = {https://www.ijbiotech.com/article_7041.html}, eprint = {https://www.ijbiotech.com/article_7041_c62751bfbf62526834d2a0a9c3de9db6.pdf} } @article { author = {Naghoosi, Hamed and Behzadian, Farida and Saeedinia, Alireza and Ghorashi, Seyed Ali}, title = {Site-Directed Mutagenesis in Human Granulocyte-colony Stimulating Factor, Cloning and Expression in Escherichia coli}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {229-234}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Human granulocyte colony stimulating factor (hG-CSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. So far, different researchers have tried to enhance hG-CSF biological activity and stability. In this study, Polymerase Chain Reaction (PCR) based site-directed mutagenesis was performed on hG-CSF cDNA. The final amplified DNA fragment was cloned into the pBluescript sk(-) plasmid and after verification of the desired mutations by sequencing, it was subcloned into the pET-21a(+) vector and expressed in Escherichia coli BL21. The mutant G-CSF product was analyzed by SDS-PAGE and Western-blot analyses. The results show that the recombinant mutant G-CSF has been cloned and expressed successfully in prokaryotic system. This research aimed to produce a new recombinant hG-CSF expected to show enhanced biological characteristics in contrast to those of the native hG-CSF. The analysis of its function and biological characteristics remain to be examined.}, keywords = {Granulocyte colony stimulating factor (G-CSF),Site-directed mutagenesis,Expression}, url = {https://www.ijbiotech.com/article_7044.html}, eprint = {https://www.ijbiotech.com/article_7044_6cfb61ad20766f62e1d4f80a35f1b5a6.pdf} } @article { author = {Ebrahimi, Seyed Mahmoud and Aghaiypour, Khosrow and Nili, Hassan}, title = {Sequence Analysis of M2 Gene of Avian Influenza Virus Strain (A/Chicken/Iran/101/98 (H9N2)) as an Oil Vaccine Seed}, journal = {Iranian Journal of Biotechnology}, volume = {6}, number = {4}, pages = {235-138}, year = {2008}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {In this study, the full-length M2 gene of the avian influenza virus (H9N2) was isolated, analyzed and studied in detail. Total RNA was extracted and cDNA of the M2 mRNA was obtained by reverse transcriptase polymerase chain reaction (RT-PCR) using random hexamer oligoes; specific primers were used for amplification of the M2 open reading frame (ORF) region. PCR was able to amplify the desirable fragment (294-bp) of the spliced M2 gene. The nucleotide sequence homology between the Iranian isolate and other H9 and H5 subtypes of influenza A from different hosts and geographical areas deposited in GenBank ranged from 92 to 98% and the amino acid sequence homology ranged from  97 to 100%.}, keywords = {M2 gene,Sequence,RT-PCR,H9N2}, url = {https://www.ijbiotech.com/article_7045.html}, eprint = {https://www.ijbiotech.com/article_7045_e85288408b415eba6c71267614304a5f.pdf} }