@article { author = {Talebkhan, Yeganeh and Mohammadi, Marjan}, title = {Vacuolating Cytotoxin of Helicobacter pylori}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {73-81}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Vacuolating cytotoxin (VacA) is one of the most important virulence factors of H. pylori (Hp), which isthe only toxic protein that is secreted from Hp cell into the culture supernatant. The effects of VacA oneukaryotic systems is the subject of many previous and on going research studies. Intracellular targetsfor this toxin include: late endosomal and lysosomal compartments, mitochondria, cell-cell junctions andphospholipid bilayers. Its effects on these targets include vacuolation of late endosomal and lysosomalcompartments, apoptosis and channel formation, which result in the increase of ion uptake speciallyanions. The aim of this review is to increase the perception on this toxin and its functions.}, keywords = {Helicobacter pylori,Vacuolating cytotoxin,vacA,virulence,Pathogenesis}, url = {https://www.ijbiotech.com/article_6891.html}, eprint = {https://www.ijbiotech.com/article_6891_8b91d7bd3cfc332ade762275b3525e6e.pdf} } @article { author = {Arbabi, M and Alasti, F and Sanati, MH and Hosseini, S and Deldar, A and Maghsoudi, N}, title = {Cloning and Expression of Human Gamma-Interferon cDNA in E. coli}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {87-94}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be used clinically in the treatmentof various viral, neoplastic and immunosuppressed conditions or diseases. In this study, a cDNAsequence coding for human gamma interferon was synthesized from mRNA template extracted frominduced human T lymphocytes. The cDNA was then amplified by PCR, cloned in an expression vector,and transformed into Escherichia coli. The polypeptide produced through the expression of this DNAsequence in E. coli showed immunological and chemical properties resembling authentic human IFN-γ.}, keywords = {Gamma interferon,complementary DNA,Polymerase Chain Reaction,Cloning,Expression,polyclonal antibodies,Immunoblotting}, url = {https://www.ijbiotech.com/article_6872.html}, eprint = {https://www.ijbiotech.com/article_6872_a7e18f08fd6831e0ccb5869be1a81f75.pdf} } @article { author = {Eftekhar, Fereshteh and Rostamizadeh, Farkhondeh and Khodadad, Ahmad and Henry, Debora and Speert, David P.}, title = {Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {95-100}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Sixty four Iranian patients with cystic fibrosis (CF) were studied for colonization with Pseudomonasaeruginosa. The patient’s age ranged between 2 months to 18 years old. Twenty one patients werecolonized, 15 with non-mucoid and 6 with mucoid strains of P. aeruginosa. The colonization rateincreased with age and the mucoid phenotype was only recovered from the older patients. All mucoidstrains came from patients with respiratory disease whereas most of the non-mucoid isolates (n=13)were from patients with gastrointestinal disorder. The antibiograms of the isolates showed 100% sensitivityto Imipenem and Collistin followed by Ciprofloxacin (90.5%), Ceftazidime, and Tobramycin (85.7%),Amikacin, Piperacillin and Tazobactam-Piperacillin (81%), Ticarcillin (76%), Gentamycin (62%),Mezlocillin (52.4%) and Carbenicillin (43%). The MICs for Ceftazidime, Gentamycin and Tobramycinagreed with the disk test results. However, MIC determination for Amikacin showed a 100% sensitivitycompared to the disk test where 81% sensitivity was observed. The discrepancy may be due to the factthat over 20% of the isolates had borderline MIC values for Amikacin. The genomic fingerprinting of the21 isolates as well as the non-mucoid revertants ofthe mucoid strains was carried out by RAPD-PCRusing primer 272 which was previously used for typing P. aeruginosa isolates from CF patient’s. Thirteengenotypes were found among the 21 isolates. One fingerprint (A) was found in 6 patients and another(B) was shared by 2 patients, all from the same health center. The idea of the hospital as environmentalsource or cross infection between patients cannot be ruled out.}, keywords = {Pseudomonas aeruginosa,cystic fibrosis,antibiotic susceptibility,RAPD-PCR}, url = {https://www.ijbiotech.com/article_6873.html}, eprint = {https://www.ijbiotech.com/article_6873_d1cae6879e89c2c4a44d98affb394f8f.pdf} } @article { author = {Borjaliloo, Shirin and Zomorodipour, Alireza and Yakhchali, Bagher and Shojai, Sharareh}, title = {Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {101-108}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {With the aim of the production of recombinant human granulocyte macrophage colony stimulating factor  (rhGM-CSF) in the periplasmic space of Escherichia coli, the expression of hGM-CSF cDNA was examinedunder the regulation of a T7-based as well as a lac-based expression systems. For the efficient expression of hGM-CSF cDNA, the first five codons at the N-terminal were altered based on the E. coli major codon usage. The hGM-CSF cDNA, fused to pelB signal sequence, was expressed using the two inducible promoters. The expression analysis of the 2 recombinant plasmids were performed in the BL21(DE3) and TG1 strains of E. coli, respectively. After induction with 1mM isopropyl-ß-DThiogalactopyranoside (IPTG) the recombinant E.coli with T7 promoter produced hGM-CSF more efficiently than did the lac promoter. Under inducing conditionsboth of the recombinant bacteria allowed successful secretion of hGM-CSF into the periplasmic space. The optimal temperature for the over-expression of the recombinant protein under the T7-based system was 30°C and that of the lac regulated system was 28°C. The optimization of growth condition for the recombinant bacteria, produced in this work, provides mean for studying the function of environmental as well as genetic factors on the overexpression of recombinant proteins in the periplasmic space of E. coli.}, keywords = {human granulocyte macrophage colony stimulation factor,IPTG,Lactose,T7/lac,Expression system,Escherichia coli and recombinant protein}, url = {https://www.ijbiotech.com/article_6874.html}, eprint = {https://www.ijbiotech.com/article_6874_5821755181415856af090ed096a0e83d.pdf} } @article { author = {Vallian, Sadeq and Farzaneh, Farzin}, title = {Expression of Recombinant 3-Beta Hydroxysteroid Dehydrogenase Protein in E. coli}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {109-114}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {3-beta hydroxysteroid dehydrogenase (3BHSD) is secreted by the cortex of adrenal gland functioning instress conditions. The gene encoding the 3-beta hydroxysteroid dehydrogenase protein was PCRamplified from a λgt11 cDNA library using specific primers. The amplified PCR product was then cloned into pGEX-4T-1 expression vector under Ptac promoter and the expression of the enzyme was examined in E. coli (BL21). Upon optimization of the expression condition, the enzyme was produced as a glutathione S-transferase (GST) fusion protein, which was purified by affinity chromatography using glutathione sepharose column. The GST part was then removed by selective proteolytic digestion with thrombin. The purified recombinant enzyme could be used in construction of diagnostic kits for screening the patients with premature ovarian failure (POF) for the presence of autoantibodies against 3BHSD as an important molecular target.}, keywords = {3-beta hydroxysteroid dehydrogenase,Recombinant protein,autoantibody}, url = {https://www.ijbiotech.com/article_6888.html}, eprint = {https://www.ijbiotech.com/article_6888_9d31f51e8df9975184973d56da81cbd9.pdf} } @article { author = {Jonoud, S and Vosoughi, M and Khalili Daylami, N}, title = {Study on Nitrification and Denitrification of High Nitrogen and COD Load Wastewater in Moving Bed Biofilm Reactor}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {115-120}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Removing nitrogen, as one of the most common and abundant pollutant of ground and surface waters isvery important. For this purpose, biological nitrification and denitrification as the most economical method should be investigated. Feasibility of high load (Chemical Oxygen Demand) COD (1000-2000 mg/l) and NH4 (1000-2000 mg/l) wastewater treatment, at different Hydraulic Retention Times (HRTs) was studied in two 9-lit anaerobic-aerobic system in pre-denitrification mode. Moving Bed Biofilm Reactor (MBBR) is a new system, having all the advantages of activated sludge, fluidized bed and fixed bed processes, without disadvantages of each system, that the biofilm production takes place on the packings, moving along the height of the reactor. From the experiments carried out in this system, result, showed higher ammonia removals take place at higher ammonia and lower organic loads. Denitrification increases at higher nitrification rates because of theincreasing effect of NO3 - entering the anaerobic reactor. In spite of the fact that nitrifying bacteria are more sensitive than COD and NO3 - removing bacteria, after toxic shock by phenol as organic source,nitrification rate increases and COD removal decreases according to the damaging effect of phenol on COD removing bacteria. Total COD removal during the study varies between 80-100%, this value changes to 30-80% for ammonia and 30-80% for ammonia and 40-90% for nitrate.}, keywords = {Biological Treatment,Denitrification,Moving Bed Biofilm Reactor (MBBR),Nitrification,Hydraulic Retention Time (HRT)}, url = {https://www.ijbiotech.com/article_6896.html}, eprint = {https://www.ijbiotech.com/article_6896_3e1a606d5290919af850aef4678c3f71.pdf} } @article { author = {Khadem Haghighat, Firoozeh and Eftekhar, Fereshteh and Mazaheri, Mahnaz}, title = {Isolation of a Dibenzothiophene Desulfurizing Bacterium from Soil of Tabriz Oil Refinery}, journal = {Iranian Journal of Biotechnology}, volume = {1}, number = {2}, pages = {121-124}, year = {2003}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {A dibenzothiophene (DBT) degrading bacterium, which utilizes DBT as the sole source of sulfur, was isolated from soil contaminated with crud oil collected from oil refinery of Tabriz (Iran). A convenient spectrophotometric assay (Gibbs’ assay) was used to determine the quantity of desulfurized product(Hydroxybiphenyl). This isolate did not grow on DBT, dibenzothiophene sulfone (DBTO2), or 2-Hydroxybiphenyl (HBP) as sole carbon sources. Biodesulfurization activity was observed only in growingcultures and depressed by free sulfate. The desulfurization trait was expressed at increasing levels during the exponential phase of growth and then declined in stationary-phase cells. This gram-positive, non-spore-forming, partially acid fast, polymorphic bacterium with the ability to desulfurize DBT or DBTO2 was identified as Rhodococcus sp. and designated strain FMF.}, keywords = {isolation,Biodesulfurization,Dibenzothiophene,Rhodococcus sp}, url = {https://www.ijbiotech.com/article_6893.html}, eprint = {https://www.ijbiotech.com/article_6893_22c99ee528362347341421a5f2e18688.pdf} }